燕麦麸皮多酚对四氧嘧啶致糖尿病小鼠的保护作用研究

Protective Effects of Oat Bran Polyphenols in Alloxan-Induced Diabetic Mice

摘要:

以四氧嘧啶建立糖尿病小鼠模型，研究燕麦麸皮多酚对糖尿病小鼠的一般生活行为、体重、空腹血糖、糖耐量试验血糖、血清胰岛素、脏器系数（肝、肾、脾和胸腺）及肝中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙酮酸激酶（PK）、己糖激酶（HK）和糖元的影响。结果表明，与模型组相比高剂量燕麦麸皮多酚组[120 mg/(kg?d)]糖尿病小鼠的体重，脾和胸腺的脏器系数，血胰岛素，肝中HK、SOD的活性及糖元含量显著增加（分别为21.53%、53.85%、54.54%、31.77%、48.69%、42.58%和84.05%）；减轻其多食、多饮和多尿的症状；糖尿病小鼠的空腹血糖显著降低39.54%，糖耐量试验0.5、2 h后血糖分别显著降低20.20%和36.78%，肝中MDA的含量显著降低35.04%。燕麦麸皮多酚对四氧嘧啶致糖尿病小鼠有保护作用。

Abstract:

Alloxan was used to establish a mouse model of diabetes. The effects of oat bran polyphenols on the general life behavior, body weight, fasting blood glucose, glucose tolerance, serum insulin, and organ indices (liver, kidneys, spleen, and thymus) of diabetic mice were studied. The malondialdehyde (MDA) and glycogen content and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), pyruvate kinase (PK), and hexokinase (HK) in the mouse liver were also recorded. The results indicated that, compared with the model group, the high-dose oat bran polyphenol group (120 mg/kg/d) showed significantly higher body weight, splenic index, thymus index, serum insulin, activities of SOD and HK in the liver, and liver glycogen content, with increases of 21.53%, 53.85%, 54.54%, 31.77%, 48.69%, 42.58%, and 84.05%, respectively. High doses of oat bran polyphenols noticeably reduced the symptoms of excessive drinking, eating, and urinating. In the high-dose oat bran polyphenol group, the fasting blood glucose, blood glucose levels at 0.5 and 1.0 h after glucose tolerance test, the content of MDA in the liver were also decreased significantly by 39.54%, 20.20%, 36.78%, and 35.04%, respectively. Oat bran polyphenols have protective effects in mice with alloxan-induced diabetes.