山茱萸中马钱苷和莫诺苷对HepG2细胞及其胰岛素抵抗模型的影响研究

Effects of Loganin and Morroniside Extracted from Cornus officinalis on HepG2 Cells and Their Insulin Resistance Model

摘要:

本文通过微波辅助提取、有机溶剂萃取、大孔树脂、制备色谱和HPLC等方法对山茱萸活性成分进行分离、纯化和鉴定，得到马钱苷和莫诺苷两种主要成分。考察了正丁醇30%乙醇洗脱相（Fr. Bu-2）、马钱苷、莫诺苷对HepG2细胞及其胰岛素抵抗模型摄取葡萄糖的影响以及对HepG2细胞的毒性作用，结果发现，马钱苷和莫诺苷对HepG2细胞增殖没有显著影响；Fr. Bu-2、马钱苷和莫诺苷均能显著促进HepG2细胞葡萄糖摄取（GU），其中马钱苷促进作用最强，且呈剂量依赖性，在0.5 mg/mL浓度时，马钱苷组和莫诺苷组GU分别是对照组的3.24倍和1.64倍；此外，Fr. Bu-2、马钱苷、莫诺苷在高浓度（?0.1 mg/mL）下均能显著促进胰岛素抵抗模型细胞GU，在0.5 mg/mL浓度时，马钱苷组和莫诺苷组的模型细胞GU分别为对照组的1.76倍和1.27倍。这些结果说明Fr. Bu-2、马钱苷和莫诺苷均能通过促进HepG2细胞及其胰岛素抵抗模型摄取葡萄糖发挥降血糖作用。

Abstract:

Microwave-assisted extraction, organic solvent extraction, macroporous resin chromatography, preparative chromatography, and high-performance liquid chromatography (HPLC) were used to isolate, purify, and identify active ingredients from Cornus officinalis. Two main types of iridoid glycosides-loganin and morroniside-were isolated, and the fraction eluted using n-butanol 30% ethanol (Fr. Bu-2) was used to investigate the effects of loganin and morroniside on glucose uptake (GU) by HepG2 cells and a HepG2 cell insulin resistance model; their cytotoxic effects on HepG2 cells were also assessed. The results showed that loganin and morroniside had no significant effect on cell proliferation. Fr. Bu-2, loganin, and morroniside could significantly promote GU by HepG2 cells. Among them, loganin exhibited the strongest promotion effect in a dose-dependent manner. The GU with 0.5 mg/mL loganin and morroniside was about 3.24 and 1.64 times that of the control group, respectively. Moreover, high concentrations (>0.1 mg/mL) of Fr. Bu-2, loganin, and morroniside could significantly promote GU by HepG2 insulin resistance model cells, especially loganin. The GU with 0.5 mg/mL loganin and morroniside was about 1.76 and 1.27 times that of the control group, respectively. In summary, Fr. Bu-2, loganin, and morroniside can exert a hypoglycemic effect by promoting GU by HepG2 cells and their insulin resistance model.