母体铅暴露对仔鼠肾脏中MMP-2、MMP-9 mRNA表达的影响

Effect of Maternal Lead Exposure on MMP-2 and MMP-9 mRNA Expression in the Kidneys of Mouse Offspring

摘要:

为了探讨母体铅暴露对仔鼠肾脏中MMP-2、MMP-9 mRNA表达的影响。采用自由饮水模式建立铅暴露动物模型，将40只雌性小鼠自妊娠第1 d开始经饮水染铅(1.0 g/L、5.0 g/L和10.0 g/L，对照组饮蒸馏水)至仔鼠出生后21 d，分别测定其血液和肾脏组织中铅的含量，然后取其肾脏组织，通过实时荧光定量PCR (Real-time PCR)的方法检测MMP-2和MMP-9 mRNA的表达情况。21 d仔鼠血液中铅水平均明显高于对照组(p<0.05)；低、中和高剂量铅暴露组仔鼠肾脏组织中MMP-2 mRNA的表达明显高于对照组(p<0.05)，铅暴露组仔鼠肾脏组织中MMP-2 mRNA的表达与对照组相比差异不显著(p>0.05)。与对照组相比，染毒组仔鼠肾脏内MMP-9 mRNA的表达明显高于对照组，且随染毒剂量增加，表达量增加，差异具有统计学意义(p<0.05)母体铅暴露使铅在仔鼠体内蓄积，可能通过增强仔鼠肾脏中MMP-2和MMP-9 mRNA的表达进而造成肾脏损伤，引起神经毒性。

Abstract:

To explore the effect of maternal lead exposure on matrix metalloproteinase (MMP)-2 and MMP-9 mRNA expression in the kidney of mouse offspring, an animal model was established using lead-contaminated drinking water. A total of 40 female mice were given free access to water containing lead acetate solutions at dosages of either 1.0 g/L, 5.0 g/L, or 10.0 g/L from day 1 of pregnancy to postnatal day 21. A control group was given distilled water. The contents of lead in blood and kidneys were measured, and the kidney tissues were collected to detect the expression of MMP-2 and MMP-9 mRNAs by real-time fluorescent quantitative polymerase chain reaction (real-time PCR). The results showed that compared with the control group, the lead levels in the blood and kidneys of 21-day-old mouse pups in the lead-treated groups were significantly higher (p<0.05). Real-time PCR showed that the expression of MMP-2 mRNA in the kidneys of low, medium, and high lead exposure groups was higher than that of the control group (p<0.05), but no significant differences were found between the expression of MMP-2 mRNA in the kidneys of the lead exposure group and those of the control group (p>0.05). The MMP-9 mRNA expression in kidneys from the lead-exposed groups was significantly higher than that of the control group; the expression level increased with increasing dose, and the difference was statistically significant (p<0.05). Maternal lead exposure could induce lead accumulation in the mouse offspring, and kidney damage and neurotoxicity could be caused, most likely due to the enhancement of MMP-2 and MMP-9 mRNA expression in the kidneys of the offspring.