黑果枸杞花青素对Aβ42致痴呆模型大鼠记忆力及抗氧化活性研究

Memory Enhancing and Antioxidant Activities of Lycium ruthenicum Murray Anthocyanin Extracts in an Aβ42-Induced Rat Model of Dementia

摘要:

采用双侧海马CA1区注射Aβ42构建阿尔茨海默症(Alzheimer′s disease，AD)动物模型，通过穿梭箱被动回避记录行为学数据；以大鼠血清和脑组织中的总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活力及还原型谷胱甘肽(GSH)、丙二醛(MDA)和蛋白质羰基(PC)含量作为评价指标，分析黑果枸杞花青素(OPC)样品对AD模型大鼠记忆力以及体内抗氧化活性的影响。结果表明，模型组动物的记忆能力显著下降，而黑果枸杞花青素组可改善AD模型大鼠的记忆损伤；同时，发现灌胃剂量为80 mg/kg的黑果枸杞花青素组能显著提高AD大鼠血清和脑组织中T-SOD、CAT活力和GSH含量，并降低MDA和蛋白质羰基含量水平。综合上述结果可知，本研究所述黑果枸杞花青素具有良好的增强体内抗氧化活性和提高AD大鼠记忆力的作用，并具有预防AD的潜在功效。

Abstract:

Amyloid beta-protein 1-42 (Aβ42) was injected bilaterally into the hippocampal CA1 area of rats to produce an animal model of Alzheimer′s disease (AD), and animal behavior was assessed using the passive-avoidance test in a shuttle box. The activities of total superoxide dismutase (T-SOD) and catalase (CAT), and the contents of glutathione (GSH), malondialdehyde (MDA), and protein carbonyl (PC) in serum and brain tissues were used as evaluation indicators to investigate the effect of Lycium ruthenicum Murray anthocyanin extracts on the in vivo antioxidant activity and memory function of AD model rats. The results showed that the memory capacity of the model group was significantly decreased, while the memory impairment of rats administered Lycium ruthenicum Murray anthocyanin extracts was alleviated. Additionally, the activities of T-SOD and CAT, and the content of GSH in serum and brain tissues were significantly enhanced, whereas MDA and PC contents were decreased in the group that received 80 mg/kg Lycium ruthenicum Murray anthocyanin extracts. Thus, Lycium ruthenicum Murray anthocyanin extracts could enhance memory capacity and antioxidant activities of AD model rats, and had a potential preventive effect against AD.