植物乳杆菌35对大肠杆菌在肠上皮细胞HT-29上黏附及刺激产生促炎细胞因子的影响

Effect of Lactobacillus plantarum 35 on Escherichia coli-induced IL-8 Production and E. coli Adhesion on HT-29 Cells

摘要:

本论文采用肠上皮细胞模型HT-29细胞系研究8株植物乳杆菌在HT-29细胞上的黏附性，并研究黏附性较强的菌株及其胞外多糖（ExopolySaccharides，EPS）抑制大肠杆菌(E?coliATCC25922)在HT-29细胞上黏附及刺激HT-29细胞产生炎性因子的作用。结果表明8株植物乳杆菌在HT-29细胞上的黏附性差异较大：黏附性最强的植物乳杆菌35通过取代、竞争和排阻方式抑制大肠杆菌在HT-29细胞上黏附，抑制黏附率分别为30%、33%和59%，其EPS在作用浓度为500 μg/mL时对大肠杆菌的抑制黏附率为32%。植物乳杆菌35可抑制大肠杆菌刺激HT-29细胞产生IL-8，通过取代、竞争、排阻方式抑制大肠杆菌刺激HT-29细胞产生IL-8，抑制率分别为3%、28%和40%；其EPS抑制大肠杆菌刺激HT-29细胞产生IL-8具有浓度效应，浓度为500 μg/mL时抑制率最高，为50%。而对于IL-10表达量的影响均不显著。结果表明植物乳杆菌35具有抑制大肠杆菌引起肠炎的潜在益生功能。

Abstract:

The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line, and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides (EPS) on Escherichia coli-induced IL-8 production and E. coli adhesion on HT-29 cells were investigated. The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees, and L. plantarum 35 had the highest adhesive ability. L. plantarum 35 could protect HT-29 cells from E. coli adhesion by displacement, competition, and exclusion, with inhibition rates of 30%, 33%, and 59%, respectively. At an active concentration of 500 μg/mL, EPS could inhibit E. coli adhesion by 32%. L. plantarum 35 could inhibit E. coli-induced IL-8 production by HT-29 cells by displacement, competition, and exclusion with inhibition rates of 3%, 28%, and 40%, respectively. EPS could inhibit E. coli-induced IL-8 production by HT-29 cells in a dose dependent manner, with the highest inhibitory rate of 50% at 500 μg/mL concentration. L. plantarum 35 had no effect on IL-10 production by HT-29 cells. The results showed that L. plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E. coli.