茶黄素与真菌多糖联合清除ABTS自由基活性的比较

ABTS Radical Scavenging Activity of Theaflavins Combined with Fungal Polysaccharides

摘要:

本文分别比较了茶黄素（TFs）与黑木耳多糖（AAP）和灵芝孢子多糖（GLP）单一、联合使用清除ABTS自由基活性的作用。采用Chou-Talaly联合指数（CI）方法分析ABTS自由基清除实验结果，并对单一和联合使用的清除效果、联合指数（CI）、等效应图和剂量减少指数（DRI）进行分析评价。单独使用茶黄素、黑木耳多糖和灵芝孢子多糖的IC50值分别为20.60 μg/mL、2.49 mg/mL和0.93 mg/mL，联合使用茶黄素和黑木耳多糖与茶黄素和灵芝孢子多糖（质量比为1:3）后，IC50值分别为74.04 μg/mL和58.92 μg/mL。联合指数（CI）分析表明，从清除率5%到97%，茶黄素和灵芝多糖的联合指数都小于1，且CI有降低趋势，二者间有协同作用；剂量减少指数（DRI）分析显示，从清除率5%到97%，茶黄素和灵芝多糖的剂量减少指数都大于1，以上结果茶黄素和黑木耳多糖联合使用均表现不一致。茶黄素和灵芝多糖之间存在协同清除ABTS自由基效应，但茶黄素和黑木耳多糖不存在。

Abstract:

The 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of theaflavins (TFs) alone and combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP) were compared in this paper. The results of ABTS radical scavenging experiments were analyzed using the Chou-Talalay combination index (CI), and the scavenging effects, combination index (CI), and dose-reduction index (DRI) of TFs, AAP, and GLP, alone and in combination, were evaluated. The half maximal inhibitory concentration (IC50) values of TFs, AAP, and GLP were 20.60 μg/mL, 2.49 mg/mL, and 0.93 mg/mL, respectively, while the IC50 values of a combination of TFs and AAP (mass ratio 1:3) and a combination of TFs and GLP (mass ratio 1:3) were 74.04 μg/mL and 58.92 μg/mL, respectively. CI analysis indicated that with the clearance rate in the range of 5~97%, the CI values of TFs with GLP were < 1 and exhibited a downward trend, suggesting a synergistic effect between TFs and GLP. DRI analysis demonstrated that with the clearance rate in the range of 5~97%, the DRI values of TFs with GLP were greater than 1. Inconsistent results were obtained in the above analyses for a combination of TFs and APP. Therefore, there was a synergistic effect between TFs and GLP for scavenging ABTS free radicals, but no similar effect was found between TFs and APP.