Abstract:In order to study the probiotic characteristics of Lactobacillus obtained from Bama centenarians, improved MRS+CaCO3 and MRS+X-gal solid culture media were used to isolate and culture the Lactobacillus from the feces of 10 Guangxi centenarians. After culturing, 19 strains of Lactobacillus were selected. Three of these strains (GU-L3, GU-P132, and GU-G23) with high tolerance to adverse gastrointestinal environments and good adhesiveness were obtained through physiological and biochemical experiments, sugar fermentation experiments, simulated gastrointestinal transit experiments, and auto-aggregation and hydrophobicity screening. Sequencing of 16S rDNA revealed that GU-L3 was Lactobacillus casei, GU-P132 was Lactobacillus fermentum, and GU-G23 was Lactobacillus gasseri. After the simulated gastrointestinal transit experiment, the survival rates of the L. casei, L. fermentum, and L. gasseri were found to be above 107 cfu/mL, while the auto-aggregation rates were 12.43±0.03%, 78.88±0.01%, and 35.92±0.02%, respectively, and the hydrophobicity rates were 6.38±0.02%, 81.77±0.02%, and 10.58±0.05%, respectively. The adhesiveness of these strains was already known to be higher than that of the other strains. Compared with the reports of related domestic and international probiotic strains, the GU-L3, GU-P132, and GU-G22 intestinal Lactobacillus strains from Bama centenarians had better probiotic characteristics and development potential.
