黄金茶醇提取物成分及抗氧化活性的研究

Study on the Constituents and Antioxidant Activity of Gold Tea Alcohol Extract

摘要:

研究了黄金茶醇提取物组成及体外抗氧化活性。采用乙醇提取、石油醚和乙酸乙酯萃取后得粗提物，并通过液相-质谱联用技术对其结构进行了初步鉴定，分析了醇提物体外抗氧化活性。结果表明，黄金茶醇提取物由绿原酸、对香豆酰奎尼酸、咖啡酸、芦丁、金丝桃苷、山奈酚-3-O-芸香糖苷、阿魏酸、圣草酚-O-己糖苷和木犀草苷所组成。黄金茶醇提物具有较好的体外抗氧化能力：在相同的浓度下，相对于Vc来说，对DPPH自由基具有更好的清除能力；对羟自由基有较好的清除能力，随浓度的增大，其清除能力逐渐增大；对超氧阴离子具有较强清除能力，浓度为250 μg/mL时，清除率可达89.80%；在50~100 μg/mL浓度下，对ABTS+自由基的清除能力比Vc效果更好，在100~250 μg/mL浓度下，两者的清除效果相当；相同浓度下，醇提物的还原能力比Vc稍弱。

Abstract:

The in vitro anti-oxidant activity and the constituents of gold tea ethanol extract were investigated. The structure of the crude extract was examined using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after ethanol extraction as well as petroleum ether and ethyl acetate extraction. The anti-oxidant activity was then analyzed in vitro. The results showed that gold tea ethanol extract was composed of chlorogenic acid, p-coumaroylquinic acid, caffeic acid, rutin, hyperin, kaempferol-3-O-rutinoside, ferulic acid, eriodictyol-O-hexoside, and luteoloside. The ethanol extract was found to exhibit a high antioxidant capacity in vitro. At the same concentration, the DPPH radical scavenging activity of the ethanol extract was higher than that of Vitamin C (Vc). The extract also had a high hydroxyl radical scavenging ability, which increased in a dose-dependent manner. In addition, the extract had the ability to scavenge superoxide anions, and this ability reached 89.80% at a concentration of 250 μg/mL. When the ABTS+ radical scavenging activity was measured, the ethanol extract was found to have higher activity than Vc, when the concentration was 50~100 μg/mL; when the concentration was 100~250 μg/mL, the scavenging activity of the two were comparable. At the same concentration, the reducing capacity of the extract was slightly lower than that of Vc.