蓝莓叶槲皮素和金丝桃苷的HPLC-MS鉴定及在内皮细胞中的舒血管和抗炎作用研究

Vasodilator and Anti-inflammatory Effects of Quercetin and Hyperin from Blueberry Leaves Identified by HPLC-MS in Umbilical Vein Endothelial Cells

摘要:

通过建立体外肿瘤坏死因子（TNF-α）诱导人脐静脉血管内皮细胞（HUVEC）炎症损伤反应模型，研究蓝莓叶中槲皮素及其衍生物金丝桃苷对人脐静脉内皮细胞中血管紧张素Ⅰ转化酶(ACE)以及炎症因子（单核细胞趋化蛋白-1、细胞间粘附分子-1和血管细胞间粘附分子-1）含量的影响，采用酶联免疫吸附剂测定方法（ELISA）检测细胞上清液中可溶性ACE和炎症因子蛋白含量的变化，探讨二者在血管内皮细胞中的舒血管和抗炎作用。研究结果表明，肿瘤坏死因子TNF-α可引起HUVEC内ACE和炎症因子含量显著增加，槲皮素和金丝桃苷预处理内皮细胞能显著抑制ACE酶活性和炎症因子含量的增加(p<0.05)，具有一定的抗炎和舒血管作用。

Abstract:

TNF-α induction was used to establish an in vitro inflammatory injury model in human umbilical vein endothelial cells (HUVECs). The effects of quercetin and hyperin from blueberry leaves on angiotensin I converting enzyme (ACE) level and the content of inflammatory factors (monocyte chemoattractant protein-1, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1) in HUVECs was investigated. Soluble ACE and inflammatory factor protein content in supernatants was detected by ELISA to explore the vasodilator and anti-inflammatory function of quercetin and hyperin in endothelial cells. The results showed that the content of ACE and inflammatory factor soluble proteins was significantly increased in TNF-α-induced cells. The protein expression of ACE and inflammatory factors in HUVECs were significantly decreased (p<0.05) when the cells were treated with quercetin or hyperin, showing that both exhibited anti-inflammatory and vasodilator effects.