基于印迹聚吡咯膜电化学传感器检测S-卵白蛋白的研究

Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole Film for Detection of S-ovalbumin

摘要:

鉴于蛋清中S-卵白蛋白的含量与鸡蛋新鲜度和品质有较大相关性，将S-卵白蛋白含量作为评价鸡蛋新鲜度和品质的指标具有较大的前景和意义。本文通过循环伏安法和差分脉冲伏安法对传感器制备的扫描圈数、扫描速率、模板洗脱时间和孵化时间进行了优化，确定最佳扫描圈数为18，扫描速率为100 mV/s，模板洗脱时间为1.5 h，孵化时间为40 min，成功制备了能特异性识别S-卵白蛋白的印迹聚吡咯膜电化学传感器。采用场发射扫描电镜对印迹和非印迹传感器的微观结构进行了表征。通过差分脉冲伏安法，探讨了不同S-卵白蛋白浓度与峰电流变化的依赖关系，确定S-卵白蛋白浓度在10-6~10-3 mg/mL范围内时，浓度与峰电流变化值具有良好线性关系，并得出检出限为6.75×10-7 mg/mL。该印迹传感器对S-卵白蛋白具有良好特异性性响应，可用于定量检测S-卵白蛋白。

Abstract:

Since the S-ovalbumin content in egg white has a significant correlation with the freshness and quality of chicken eggs, S-ovalbumin can be consider as a useful indicator for the same. Conditions were optimized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) to prepare a molecularly-imprinted polypyrrole (MIPs) sensor that selectively recognizes S-ovalbumin. The optimal scan cycles, scan rate, template extraction time, and incubation time were 18 cycles, 100 mV/s, 1.5 h, and 40 min, respectively. The micro-structure of MIPs and non-MIPs were characterized by field emission scanning electron microscopy (FE-SEM). DPV was used to evaluate the dependency between different concentrations of S-ovalbumin and changes in peak current. The results showed that the linear response range of MIPs was from 10-6 to 10-3 mg/mL with a detection limit of 6.75×10-7 mg/mL. The MIPs exhibited a selective response to S-ovalbumin, and can therefore be used in the detection of S-ovalbumin.