不同粉碎处理对香菇菌伞多酚细胞抗氧化及抗增殖活性的影响

Effects of Grinding Methods on the Antioxidant and Antiproliferative Activities of Phenolics in Mushroom Caps (Lentinus edodes)

摘要:

本研究采用三种粉碎方式处理香菇菌伞，制得样品普通粉碎香菇伞粉（Coarse milled cap，CMC）、气流超微粉碎香菇伞粉（Jet milled cap，JMC）和纳米超微粉碎香菇伞粉（Nano-micronized cap，NMC），测定不同粉碎处理的香菇伞粉多酚含量，并研究多酚对HepG2细胞抗氧化及抗增殖活性的影响。结果发现，JMC中游离酚含量最高（4.457±0.076 mg/g），结合酚含量最低（0.274±0.018 mg/g）；CMC游离酚含量最低，但结合酚含量却最高。游离酚的细胞抗氧化活性，不使用PBS清洗时其CAA值为：CMC-F>JMC-F>NMC-F；使用PBS清洗时其CAA值为：NMC-F>JMC-F>CMC-F。多酚的细胞抗增殖活性，三种粉碎处理的样品游离酚和结合酚均具有较好的抗增殖活性，EC50大小顺序均为NMC>CMC>JMC。因此，不同粉碎方式处理对香菇伞粉多酚含量、细胞抗氧化及抗增殖活性均有一定影响，但经超微粉碎处理后能显著提高总酚和游离酚含量，减少结合酚含量，而且能提高其细胞抗氧化及抗增殖活性。

Abstract:

Three grinding methods were employed on mushroom caps (Lentinus edodes) to generate three kinds of powders, coarse milled cap (CMC), jet milled cap (JMC), and nano-micronized cap (NMC). The polyphenolic content in these three samples and the effect of their antioxidant and antiprofilerative activities on HepG2 cells were determined. The results showed that JMC had the highest amount of free phenolics (4.457±0.076 mg/g) and the lowest amount of bound phenolics (0.274±0.018 mg/g), while CMC had the highest amount of bound phenolics and the lowest amount of free phenolics. The results of the cellular antioxidant activity (CAA) assay showed that the order of CAA values after the three treatments without washing with PBS was: free phenol in CMC (CMC-F) > free phenol in JMC (JMC-F)>free phenol in NMC (NMC-F). The order of CAA values after washing with PBS was: NMC-F > JMC-F > CMC-F. The results of the cell proliferation assay indicated that the free phenolics and bound phenolics after all three treatments had significant effects on inhibiting the proliferation of HepG2 cells. The order of EC50 values was: NMC>CMC>JMC. Therefore, different grinding methods had distinct effects on the polyphenolic content, antioxidant activity, and antiproliferation activity of Lentinus edodes caps. Super?ne grinding could significantly increase the production of total phenolics and free phenolics, decrease the content of bound phenol, and improve the antioxidant activity and antiproliferation activity of Lentinus edodes.