人工模拟肠炎沙门氏菌在绿豆芽中的内化定殖能力及其消毒处理研究

Internalization Capacity of Salmonella Enteritidis in Mung Bean Sprouts using Artificial Simulation and its Disinfection

摘要:

本研究以绿豆为作用载体，研究了肠炎沙门氏菌（ATCC13076）在绿豆芽的不同发芽阶段中的内化定殖能力及消除绿豆芽中定殖的肠炎沙门氏菌的有效方法。结果表明：在绿豆发芽前期的4个不同时段（0~48 h内）：吸胀期（G1，0 h）、萌动期（G2，12 h）、发芽初期（G3，24 h）、发芽期（G4，48 h），分别接种102、104、106和108 CFU/mL的肠炎沙门氏菌时，其在绿豆芽中的内化定殖能力不同。接种浓度为102 CFU/mL左右时，肠炎沙门氏菌在发芽初期（G3）和发芽期（G4）的内化能力较强，另外两个阶段比较微弱。接种浓度不低于104 CFU/mL时，肠炎沙门氏菌在吸胀期（G1）的内化能力最强。在吸胀期（G1）接种108 CFU/mL的菌液时，最高内化量可达2.6×108 CFU/g。紫外照射消毒处理对内化定殖的肠炎沙门氏菌有明显的去除效果，而次氯酸钠溶液和硝酸银溶液浸泡处理对内化的肠炎沙门氏菌的去除效果并不明显。

Abstract:

The artificial contamination method was used to study internalization and colonization capacities of Salmonella Enteritidis (ATCC13076) in mung bean sprouts during different stages of germination. Disinfection treatments for internalized Salmonella Enteritidis were also developed. During the four stages (0~48 h) in the pre-germination stage of mung beans (the expansion period [G1, 0 h], the budding period [G2, 12 h], early germination [G3, 24 h], and germination [G4, 48 h]), Salmonella Enteritidis was found to possess different internalization and colonization capacities when inoculated at different concentrations (102, 104, 106, and 108 CFU/mL). When inoculated at a concentration of 102 CFU/mL, Salmonella Enteritidis showed the strongest internalization capability in the germination stage and the seedling stage and at the other two stages, the internalization was relatively weak. With inoculation at a concentration of 104 CFU/mL, Salmonella Enteritidis showed the strongest internalization capacity in the imbibition stage, and with inoculation at a concentration of 108 CFU/mL, the maximum amount of internalized Salmonella Enteritidis in the imbibition stage increased to 2.6×108 CFU/g. Disinfection by ultraviolet radiation can effectively eliminate internalized Salmonella Enteritidis, whereas dip-treatment in sodium hypochlorite solution and silver nitrate solution had no obvious effect.