不同生理状态下人参总皂苷对HepG2细胞CYP2B6 mRNA和蛋白表达的影响

Effects of Total Ginsenosides on Expression of CYP2B6 mRNA and Protein in HepG2 Cells in Normal and Inflammatory States

摘要:

本文研究人参总皂苷在正常和炎症状态下对HepG2细胞CYP2B6 mRNA和蛋白表达的影响。采用索式提取法提取人参总皂苷，Real-Time PCR及Western Blot技术检测细胞中CYP2B6 mRNA和蛋白表达。结果显示，正常状态下人参总皂苷低（15 g/mL）、中（60 g/mL）与高（240 g/mL）剂量均显著诱导CYP2B6 mRNA表达（p<0.05），中、高剂量均显著诱导CYP2B6蛋白的表达（p<0.05）。使用LPS孵育细胞制造炎症模型，LPS对CYP2B6 mRNA表达无影响，但显著诱导CYP2B6蛋白的表达（p<0.05）。使用人参总皂苷和LPS共同孵育细胞后，炎症状态下人参总皂苷中、高剂量对CYP2B6 mRNA的表达仍有显著诱导效果（p<0.05），低和中剂量则均能显著诱导CYP2B6蛋白的表达，但高剂量则对CYP2B6的蛋白表达则呈现抑制作用（p<0.05）。提示炎症状态能够改变人参总皂苷对CYP2B6 mRNA和蛋白的表达，因此联合用药时，应考虑不同生理状态下人参对代谢性药物相互作用的影响。

Abstract:

The purpose of this study was to determine the effects of total ginsenosides on the expression of CYP2B6 mRNA and protein in HepG2 cells during normal and inflammatory states. Total ginsenosides were extracted using the Soxhlet extraction method and colorimetric analysis was employed to measure the concentration of total ginsenosides. The expression levels of CYP2B6 mRNA and protein were detected by RT-PCR and western blot, respectively. The results showed that in the normal state, low, medium, and high concentrations of total ginsenosides could induce the expression of CYP2B6 mRNA in HepG2 cells (p<0.05), and medium and high concentrations could induce CYP2B6 protein expression (p<0.05). LPS treatment of HepG2 cells could significantly increase CYP2B6 protein levels (p<0.05) but had no effect on CYP2B6 mRNA. Compared with the normal state, medium and high concentrations of total ginsenosides could induce the expression of CYP2B6 mRNA after LPS treatment (p<0.05). Low and medium concentrations of total ginsenosides could induce expression of CYP2B6 protein, while the high concentration had an inhibitory effect in LPS-treated HepG2 cells (p<0.05). These results suggested that inflammation state can change the mRNA and protein expression of CYP2B6 induced by total ginsenosides. Therefore, drug-drug interaction between Panax ginseng and other drugs during the normal physiological state and LPS infection should be considered.