Abstract:The Illumina HiSeq 2500 sequencing (RNA-Seq) method was used to analyze the transcriptome of Rehmannia glutinosa virus-free seedlings in this paper. The transcriptome information was obtained, and protein functional annotation, Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway analysis, and other bioinformatics analyses were conducted on the constructed R. glutinosa Unigene database, in order to deeply explore the genes related to biosynthesis and modification of iridoids in R. glutinosa. High-throughput sequencing technology was used for transcriptome sequencing analysis in R. glutinosa seedlings, generating 13.61 GB of clean data, and 70,778 unigenes were obtained after de novo assembly. Among them, 33,428 unigenes (47.23%) could be annotated in public non-redundant (Nr) databases, including the Swiss protein database (Swiss-Prot), protein families (Pfam), eukaryotic orthologous groups (KOG), KEGG, gene ontology (GO), and cluster of orthologous groups (COG). The transcripts encoding known enzymes involved in biosynthesis and post modification of terpenoids were discovered in the Illumina dataset, and 81 unigenes involved in terpenoid backbone biosynthesis were identified. Additionally, 226 unigenes might be involved in the cytochrome P450 (CYP450)-mediated oxidative modification of iridoid backbones, and 102 unigenes were associated with uridine diphosphate (UDP)-glycosyltransferases (UGTs). The transcriptome data from this study lays a basis for the study of the biosynthesis of iridoids and provides a reference for the follow-up analysis of gene functions and other studies.
