密蒙花黄酮对四氯化碳所致小鼠急性肝损伤的保护作用

Heptoprotective Effects of Flavonoids from Buddleja officinalis Maxim. (L.) DC. against CCl4-induced Acute Hepatic Damage in Mice

摘要:

研究70%的乙醇浸提的密蒙花黄酮提取物对四氯化碳(CCl4)所致小鼠肝损伤的保护作用。密蒙花黄酮50、100 mg/(kg?d)剂量能显著抑制CCl4所致肝损伤小鼠肝脏指数、脾脏指数，可以使CCl4诱导的肝损伤小鼠血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、胆碱酯酶(CHE)、总胆红素(TBIL)含量下降，同时，密蒙花黄酮能提高肝脏抗氧化酶SOD的活性和GSH含量，并降低肝组织中MDA的含量，且50 mg/(kg?d)浓度密蒙花黄酮的效果更显著，接近阳性对照药物水飞蓟的作用。组织病理切片证明密蒙花黄酮可以减轻CCl4对肝组织的破坏，改善肝脏组织的病理损伤。其保护机制可能与清除自由基、抑制脂质过氧化有关。

Abstract:

The hepatoprotective effects of flavonoids of Buddleja officinalis Maxim. (L.) DC. (BMF) on CCl4-induced liver damage in mice were studied. The flavonoids extraction was carried out using 70% ethanol. The results showed that BMF extracts at doses 50 and 100 mg/kg/d significantly lowered the levels of serum alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), cholinesterase (ChE), lactate dehydrogenase (LDH), total bilirubin (TIBL), and hepatic malondialdehyde (MDA). In addition, the elevated values of liver index and spleen index induced by CCl4 (p<0.05) were suppressed by the BMF extracts. Moreover, the extracts enhanced superoxide dismutase (SOD) activity (p<0.05) and glutathione (GSH) levels (p<0.05) significantly compared to those of the model control group. Furthermore, 50 mg/kg/d BMF showed a stronger effect, which was close to that of silymarin (positive control drug). The results of the histopathological section demonstrated that BMF could mitigate the CCl4-induced damage in the liver tissues, thereby reducing the extent of pathological damage. The protective mechanism of BMF is possibly related to the free radicals scavenging activity and the inhibition of lipid peroxidation.