翡翠贻贝提取物对小鼠急性肝损伤的保护作用

Hepatoprotective Activity of Perna viridis Extracts Against CCl4-induced Liver Injury in Mice

摘要:

本文研究探讨了翡翠贻贝提取物对小鼠急性肝损伤的保护作用。建立四氯化碳（CCl4）致小鼠急性肝损伤模型，测定血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)含量及肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、肿瘤坏死因子α(TNF-α)和白介素-6(IL-6)含量；采用双向电泳分析肝脏差异表达蛋白；病理切片观察小鼠肝组织病理损伤的情况。结果表明，与模型组相比较，翡翠贻贝提取物各剂量组小鼠ALT、AST水平和MDA含量均显著降低，SOD、CAT、GSH-Px和GSH含量显著提高，TNF-α和IL-6水平也显著降低（p<0.01）。通过双向电泳获得了蛋白质显著差异点97个，鉴定出在模型组中有4个蛋白高表达，1个蛋白低表达；在翡翠贻贝提取物组中3个蛋白高表达。病理镜检显示小鼠肝脏损伤程度有所减轻。从以上实验结果可推测，翡翠贻贝提取物对CCl4引起的小鼠急性化学性肝损伤具有明显的保护作用。

Abstract:

Here, the protective effect of Perna viridis extract against acute liver injury was explored. A carbon tetrachloride (CCl4)-induced mouse model of acute liver injury was established, and levels of serum aspartate aminotransferase (AST) and alanine transaminase (ALT), as well as the content of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH), tumor necrosis factor alpha (TNF-α), and interleukin 6 (IL-6) in hepatic tissues, were determined. Two-dimensional (2D) electrophoresis was used to analyze the differentially expressed proteins in the liver, and pathological damage in liver tissue was observed by pathological sectioning. The results showed that the groups treated with P. viridis extract at different doses showed a significant decrease in serum AST and ALT levels and the content of MDA, TNF-α, and IL-6, as well as a pronounced increase in the content of SOD, CAT, GSH-Px, and GSH (p<0.01) as compared with levels observed in the model group. The 2D-electrophoresis analysis showed 97 significantly deviating protein spots, four overexpressed proteins, and one protein exhibiting low expression in the model group, and three overexpressed proteins in the P. viridis-treated group. Additionally, microscopic pathologic examination revealed decreased liver damage in the P. viridis-treated group. These results suggested that the P. viridis extract exerted adequate protective effects against CCl4-induced acute liver injury.