Caco-2细胞模型可用于预测各种途径的食品营养物质吸收，被普遍用于营养物质开发的早期快速筛选过程中。传统Caco-2单层细胞模型的建立通常需要培养21 d，本研究建立一种快速培养Caco-2单细胞层的方法以缩短实验周期、降低成本。采用向标准培养基中添加生长因子的方法培养Caco-2细胞，分别研究细胞模型跨膜电阻值（TEER）、亲水标志物的渗透性、关键蛋白基因的表达、细胞膜特征形态等评价模型的完整性。表明快速培养法（9 d）获得的Caco-2模型跨膜电阻值（TEER）> 220 Ω?cm2；漏出标志物荧光黄表观分配系数为(0.39 ± 0.15)×10-6 cm/s；肠腔侧(apical，AP)/基底侧（Basolateral，BL）碱性磷酸酶活力比值为5.481±0.5304，单细胞层极性分化明显；qRT-PCR检测细胞单层关键蛋白质（P-gp、MRP2）的mRNA表达量快速法同标准培养法无显著差异。此法仅需9 d即可形成完整Caco-2细胞模型。结论是向标准培养基中添加生长因子的Caco-2细胞模型快速培养法可推广。

The Caco-2 cell model can be used to predict nutrient absorption in various ways, and it has been widely applied to the early rapid screening process of nutritional product development. Although it usually takes 21 days to establish a conventional Caco-2 monolayer model, an accelerated Caco-2 monolayer culture system was developed in this study to shorten the experimental period and reduce costs. Caco-2 cells were cultured by adding growth factors to the standard medium, and the model integrity was evaluated by its transepithelial electrical resistance (TEER) values, permeability of hydrophilic markers, expression of key protein genes, morphological characteristics of the cell membrane, etc. The results showed that the Caco-2 model obtained by the accelerated nine-day method had a TEER value of more than 220 Ω?cm2 and an apparent partition coefficient (Papp) value of leakage marker Lucifer yellow of (0.39 ± 0.15 ) × 10-6 cm/s. The apical (AP)/basolateral (BL) alkaline phosphatase activity ratio was 5.481 ± 0.5304, showing obvious polar differentiation of the cell monolayer. A quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the mRNA expression of the key proteins (P-gp and MRP2) in the cell monolayer, and no significant difference was found between the accelerated method and the standard culture method. Using this method, a complete Caco-2 cell model can be established in only nine days, providing a viable alternative to the conventional Caco-2 monolayer model.

公益性行业（农业）科研专项（201303084）；现代农业产业技术体系（CARS-41-K23）资助项目