有机溶剂溶样-ICP-MS法测定食用花椒籽油中的无机元素

Determination of Inorganic Elements in Edible Zanthoxylum bungeanum Seed Oil by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

摘要:

建立了测定食用花椒籽油中无机组成元素含量的分析方法。选择煤油为溶剂溶解食用花椒油样品后采用电感耦合等离子体质谱（ICP-MS）法测定了其中的Be、B、Na、Mg、P、K、Ca、Ti、V、Mn、Fe、Co、Ni、Cu、Zn、As、Mo、Cd、Sn、Sb、Hg、Pb等22种无机元素。通过优化等离子体射频功率、载气流速和样品的提升量等工作参数消除了有机溶剂直接进样所引起的信号波动，选用八极杆碰撞/反应池（ORS）技术和内标法消除了分析过程中的质谱干扰和基体效应。分析结果表明，22种无机元素的检出限在0.12～16.25 ng/g之间，相对标准偏差（RSD）为1.28%~4.16%，加标回收率在90.00%~110.00%之间，方法准确可靠。食用花椒籽油中无机组成元素中P含量最高，其次是K。该方法能用于食用花椒籽油的质量控制和营养评价。

Abstract:

A method for simultaneously measuring the content of inorganic elements in edible Zanthoxylum bungeanum seed oil (EZBSO) was established. After the EZBSO sample was dissolved in kerosene, 22 elements (Be, B, Na, Mg, P, K, Ca, Ti, V, Mn, Fe, Co, Ni, Cu, Zn, As, Mo, Cd, Sn, Sb, Hg, and Pb) were determined using inductively coupled plasma mass spectrometry (ICP-MS). The signal fluctuations caused by direct injection of organic solvent were eliminated by optimizing the plasma radio frequency power, carrier gas flow rate, sample uptake rate, and other operating parameters. Octopole reaction system (ORS) and internal standard method were used to eliminate the mass spectrometry interference and matrix effect, respectively. The results indicated that the detection limits for the 22 elements were in the range of 0.012~16.25 ng/g, relative standard deviations (RSDs) ranged from 1.28% to 4.16%, and recoveries ranged from 90.00% to 110.00%; the method was accurate and reliable. The element P showed the highest concentration in the EZBSO, followed by the element K. The method can be applied for the quality control and nutritional evaluation of EZBSO.