紫甘蓝花色苷对动脉粥样硬化兔血脂的调节作用

Effect of Red Cabbage Anthocyanins in Regulating the Blood Lipid Profile of Atherosclerotic Rabbits

摘要:

本文研究了紫甘蓝花色苷（RCA）对动脉粥样硬化兔血脂的调节作用。新西兰大白兔分为正常对照组、模型对照组、RCA低、中、高剂量组和阳性对照组(n=8)。正常对照组以普通饲料喂养，其他组均以高脂饲料喂养，6周后，RCA低、中、高三个剂量组分别灌胃30、60、90 mg/(kg?d)体重的花色苷，阳性对照组灌胃5 mg/(kg?d)体重的辛伐他汀（simvastatin），模型对照组灌胃2 mL/(kg?d)体重生理盐水。实验第6周和10周分别检测兔子血清甘油三酯（TG）、总胆固醇(TC)、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）等指标，10周后取血处死，取主动脉作病理形态学观察。结果发现辛伐他汀和RCA能明显降低兔子血清TG、TC、LDL-C的含量，病理切片显示，紫甘蓝花色苷可减轻动脉粥样硬化斑块等病理损伤，揭示RCA对高血脂和动脉粥样硬化具有重要防护作用，且呈现剂量-效应关系。

Abstract:

In this study, the influence of red cabbage anthocyanins (RCAs) in regulation of the blood lipid profile of atherosclerotic rabbits was studied. New Zealand white rabbits were divided into normal control group, model control group, low-, medium-, and high-dose anthocyanin groups, and positive control (simvastatin) group (n = 8). The normal control group was fed an ordinary diet and the other groups were fed high-fat diet. After six weeks, the rats of the low-, medium-, and high-dose anthocyanin groups were administered 30, 60, and 90 mg/(kg?d) anthocyanin by gavage, respectively; the positive control group received 5 mg/(kg?d) simvastatin; and the model group received 2 mL/(kg?d) physiological saline. Serum total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein (LDL-C) were measured after 6 and 10 weeks. After 10 weeks, the rabbits were sacrificed, and the aorta was collected for pathomorphological observation. Results showed that RCA and simvastatin significantly reduced serum TC, TGs, and LDL-C content in the rabbits. Histological sections demonstrated that RCA could mitigate atherosclerotic plaque and other atherosclerotic damages, demonstrating its important, protective, and dose-dependent effects on atherosclerosis and hyperlipidemia.