长白山榛仁（Corylus heterophylla Fisch.)蛋白酶解物对小鼠免疫功能的影响

Effect of Enzymatic Hydrolysates of Hazelnut (Corylus heterophylla Fisch.) from Changbai Mountain on the Immune Function in Mice

摘要:

本文研究不同水解度的长白山榛仁蛋白酶解物对小鼠免疫功能的影响。按水解时间不同制备六种水解度榛仁蛋白酶解物(DH分别为24.16、29.69、33.22、36.33、38.08和39.57%)，给受试组小鼠经口按1.00 g/kg?bw剂量灌胃，灌胃第10、20、30 d后，以小鼠免疫脏器指数，脾淋巴细胞增殖能力，巨噬细胞吞噬活性，血清细胞因子水平(IFN-γ和IL-4)，血清碱性磷酸酶活力为指标，研究榛仁蛋白酶解物对小鼠的免疫调节功能。结果表明：水解度33.22%的榛仁蛋白酶解物只显著增加了小鼠胸腺指数（p<0.05）；水解度为36.33、38.08和39.57%的榛仁蛋白酶解物均能显著或极显著地增加小鼠免疫脏器指数、血清IFN-γ和AKP水平，增强脾淋巴细胞增殖活性和巨噬细胞吞噬活性（p<0.05或p<0.01）。结论，水解度为36.33、38.08和39.57%的榛仁蛋白酶解物具有增强小鼠免疫功能的作用。本研究为榛仁蛋白开发具有免疫活性的肽类产品提供了实验依据。

Abstract:

The effect of enzymatic protein hydrolysates with different degrees of hydrolysis (DHs) of the hazelnut from Changbai Mountain on the immune function in mice was evaluated in this study. Hazelnut protein hydrolysates with 6 different DHs (24.16%, 29.69%, 33.22%, 36.33%, 38.08%, and 39.57%) were prepared according to their hydrolysis time, and were orally (gavage) administered to the test mice groups at a dose of 1.00 g/kg body weight. Through immune organ index analysis and the spleen lymphocyte proliferation function, phagocytic activity of macrophages, cytokine levels in serum (IFN-γ and IL-4), and the activity of alkaline phosphatase in serum as indicators, the immunomodulatory effects of hazelnut protein hydrolysates in mice were studied on 10, 20, and 30 days after administration. The results demonstrated that the hazelnut protein hydrolysate with a DH of 33.22% significantly increased the thymus index of mice (p < 0.05). The hazelnut protein hydrolysates with DHs of 36.33%, 38.08%, and 39.57% also significantly increased the immune organ index, IFN-γ and AKP levels in serum, and enhanced the spleen lymphocyte proliferative activity and macrophage phagocytic activity (p < 0.05 or p < 0.01). In conclusion, hazelnut protein hydrolysates with DHs of 36.33%, 38.08%, and 39.57% could enhance the immune function in mice. This study provides an experimental basis for the development of peptide products with immune activity from hazelnut proteins.