本文旨在研究菌株接入广式腊肠延长其货架期机制。将从广式腊肠中依据其蛋白酶、脂肪酶和亚硝酸盐还原酶活性筛选出来的葡萄球菌H33B和微球菌X142B 2株菌株应用到腊肠中，首先测定各处理组中各分子量肽段的含量，然后用4种常见的抗氧化测定方法测定各处理组中小于5 ku肽段的抗氧化活性，最后对贮藏期间（25 ℃）腊肠脂肪氧化、蛋白氧化值和高铁肌红蛋白含量进行测定。结果表明接种菌株接入不仅能够增加腊肠中小分子肽的含量，还能增强对应处理组中肽的抗氧化性能；此外菌株的接入能够减缓广式腊肠贮藏期间氧化反应的程度，其中以接入混合菌组效果最明显。这些结果表明选择具有一定工艺学特性的菌株应用于广式腊肠中，能够减缓氧化反应的程度，从而有利于货架期的延长。

The aim of this study was to investigate the mechanism of extending the shelf life of Cantonese sausages by bacterial inoculation. Strains Staphylococcus H33B and Micrococcus X142B originally isolated from Cantonese sausages based on their protease, lipase, and nitrite reductase activities were used to inoculate the sausages. First, the contents of peptides with different molecular weights in each treatment group were determined. Subsequently, four common antioxidant assays were employed to determine the antioxidant activities of the peptides (<5 ku), and finally, the values for fat oxidation, protein oxidation, and metmyoglobin content were determined. The results showed that the bacterial inoculation could not only significantly increase the content of small-molecule peptides (MW < 5 ku) in the sausage but also enhance the antioxidant activities of the peptides in the treatment group. Additionally, bacterial inoculation could reduce the extent of oxidation in the Cantonese sausages during storage, and the most significant effect was observed in the product inoculated with mixed bacteria. These results indicate that the inoculation of some kinds of bacteria into Cantonese sausages can reduce the extent of oxidation, thus extending their shelf life.

公益性行业（农业）科研专项（201303082）2013-2016；广东省重大科技专项（2011A020102001）