基于体外化学与细胞模型评价鲍内脏肽粉的抗氧化活性

Evaluation of Antioxidant Activity of Polypeptides from Abalone Viscera Using In vitro Chemistry and Cell Culture Model

摘要:

本文考察了鲍内脏肽粉对二苯基苦基苯肼自由基（DPPH?）、羟自由基（?OH）、超氧阴离子自由基（O2-?）的清除能力，结果发现，鲍内脏肽粉对DPPH?、?OH和O2-?自由基具有一定的清除能力，其IC50值分别为0.87 mg/mL、7.53 mg/mL和19.41 mg/mL。采用H2O2建立体外培养人肝癌细胞（HepG2）氧化应激损伤模型，将细胞分为正常对照组，模型组、H2O2加低（1.6 mg/mL）、中（3.2 mg/mL）、高（6.4 mg/mL）剂量组，检测各组细胞存活率、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量。结果发现，1.6 mg/mL、3.2 mg/mL和6.4 mg/mL 3个剂量水平的鲍内脏肽粉能显著提高HepG2细胞的存活率、T-AOC和SOD活力，显著抑制细胞的MDA含量，且剂量-效应关系明显，对H2O2氧化损伤HepG2细胞均具有一定的修复作用。结果表明，鲍内脏肽粉具有较好的抗氧化活性。

Abstract:

The scavenging activity of abalone visceral polypeptide (AVPP) against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH?), hydroxyl radical (?OH), and superoxide anion radical (O2-?) was determined in this study. The results showed that AVPP exhibited certain free radical scavenging activity against DPPH?, ?OH, and O2-?-, with IC50 values of 0.87, 7.53, and 19.41 mg/mL, respectively. An oxidative stress injury model was established by human hepatoma cells (HepG2) treatedwith H2O2. HepG2 cells were cultured in vitro and divided into five groups: control, model, low-dose AVPP (1.6 mg/mL), medium-dose AVPP (3.2 mg/mL), and high-dose AVPP (6.4 mg/mL). Cell viability, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels in each group were measured. The results showed that the AVPP significantly increased HepG2 cell viability at doses of 1.6 mg/mL, 3.2 mg/mL, and 6.4 mg/mL. Additionally, the AVPP increased T-AOC and SOD activity and markedly reduced the MDA levels in a positive dose-response pattern. Thus, the results indicate that AVPP possesses excellent antioxidant capability.