本实验研究了褐赭色羊肚菌游离酚和结合酚体内抗氧化能力以及对HepG2细胞的毒性和抗增殖作用。采用HPLC对褐赭色羊肚菌多酚组分进行鉴定，测定多酚抗氧化能力指数（ORAC）；以人肝癌细胞HepG2为模型，测定多酚细胞抗氧化活性（CAA值）及抗增殖活性（EC50值）。结果显示：褐赭色羊肚菌游离酚和结合酚含量分别为（4.27±0.07）、（0.14±0.01）mg GAE/g DW；其ORAC值分别为（4624.52±400）、（429.41±60）μmol TE/100 g DW；CAA值分别为（15.33±0.60）、（0.37±0.01）μmol QE/100 g DW（PBS清洗）和（51.84±0.83）、（1.27±0.04）μmol QE/100 g DW（不经PBS清洗）。游离酚和结合酚浓度为125 μg/mL和70 μg/mL时，对HepG2细胞增殖抑制率分别为77.28%、66.31%，且试验浓度范围内，羊肚菌多酚对HepG2基本无细胞毒性。褐赭色羊肚菌多酚具有一定的细胞抗氧化及抗增殖活性，可将褐赭色羊肚菌作为开发此类功能营养食品的原料，为羊肚菌的进一步综合利用提供理论依据。

The in vivo antioxidant capacity of free and bound phenols in Morchella umbrina Boud. and their toxic and antiproliferative effects on HepG2 cells were investigated in this study. The polyphenolic components in Morchella umbrina Boud were identified by high performance liquid chromatography (HPLC), in addition to measuring the oxygen radical absorption capacity (ORAC). The human liver cancer cell line HepG2 was used as a model to measure cellular antioxidant activity (CAA) and antiproliferative activity (EC50 value). The results showed that the free phenol and bound phenol content of Morchella umbrina Boud was (4.27 ± 0.07) mg GAE/g DW and (0.14 ± 0.01) mg GAE/g DW, respectively. The ORAC values of free phenol and bound phenols were (4624.52 ± 400) and (429.41 ± 60) μmol TE/100 g DW, respectively. The corresponding CAA values with PBS washing were 15.33 ± 0.60 and 0.37 ± 0.01 μmol QE/100 g DW, respectively. The corresponding CAA values without PBS washing were 51.84 ± 0.83 and 1.27 ± 0.04 μmol QE/100 g DW, respectively. HepG2 cell proliferation was inhibited at a ratio of 77.28% and 66.31% at 125 μg/mL for free phenol and 70 μg/mL for bound phenol, respectively. In addition, within the concentration range of the experiment, the polyphenols from M. umbrina Boud generally showed no cytotoxicity to HepG2 cells. These results indicate that the polyphenols in M. umbrina Boud have certain antioxidant and antiproliferative effects. Thus, M. umbrina Boud could be used as a raw material to develop related types of functional foods. This study provides a theoretical basis for further utilization of M. umbrina Boud.

国家自然科学基金面上项目(31471576)；十二五国家科技支撑计划(2013BAD16B01)