液质联用仪同步检测婴幼儿配方食品中11种水溶性维生素

Simultaneous Detection of Eleven Water-soluble Vitamins in Infant forMulae Using Liquid Chromatography–mass Spectrometry

摘要:

建立高效液相色谱-串联质谱测定婴幼儿配方食品中11种水溶性维生素的同步检测方法。样品经过前处理后,采用Waters ACOUTITY UPLC HSS T3（2.1×100 mm，1.8 μm）色谱柱进行分离；流动相为0.1%甲酸的甲醇溶液和10 mmol/L甲酸铵水溶液(pH=3.5)，梯度洗脱，采用电喷雾离子源多反应监测(MRM)模式进行检测。结果表明，11种水溶性维生素在5 min内完成一个样品的分析，线性关系良好(r≥0.999)，方法检出限在0.02~3.00 ng/mL之间。在三个水平添加回收率试验，每个水平重复5次，其平均回收率在88.0%~110.0%之间，测定结果的相对标准偏差小于5%。该方法具有快速、简单、准确、灵敏等特点，可对较宽浓度范围内的多种维生素进行同步分析，有助于提高食品检测实验室的分析效率，降低成本。可用于婴幼儿配方食品中水溶性维生素的检测。

Abstract:

A novel method to simultaneously detect 11 water-soluble vitamins in infant formulae using high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) was established. The sample was pre-treated, followed by the separation using a Waters Acuity UPLC HSS T3 (2.1 × 100 mm, 1.8 μm) column by gradient elution, with 0.1% formic acid in methanol and 10 mol/L ammonium formate solution (pH = 3.5) as the mobile phase, in conjunction with electrospray ionization multiple reaction monitoring (MRM). The results showed that each sample could be analyzed within five minutes, detecting 11 water-soluble vitamins as well as exhibiting a good linear relationship (r ≥ 0.999) and a detection limit between 0.02 and 3.00 ng/mL. The recovery rate was tested at three levels with five repeats at each level. The results showed that the average recovery rate ranged between 88.0% and 110.0%, with relative standard deviation of < 5%. This method was rapid, simple, accurate, and sensitive, and could simultaneously analyze multiple vitamins within a wide concentration range. This method can therefore help to improve analytical efficiency and reduce cost of food testing laboratories, for the detection of water-soluble vitamins in infant formulae.