水提紫甘薯色素废渣中花色苷及总黄酮生物活性的研究

Biological Activity of Anthocyanins and Total Flavonoids in Pigment Extraction Residue from Ipomoea batatas L.

摘要:

本研究对水提紫甘薯色素剩余废渣中的花色苷和总黄酮进行生物活性研究和评价。采用滤纸片扩散法研究两种组分对金黄色葡萄球菌和大肠杆菌的抑制作用；选取DPPH?体系评价抗氧化活性；采用MTT法检测两种组分对人乳腺癌细胞MDA-MB-231的抑制作用。结果显示：水提紫甘薯色素废渣中花色苷和总黄酮均具有明显抑制金黄色葡萄球菌和大肠杆菌生长、清除DPPH自由基及抵抗人乳腺癌细胞MDA-MB-231生长的作用。花色苷和总黄酮对两种供试菌的最小抑菌浓度分别为400 μg/mL和4.0 mg/mL；花色苷对DPPH自由基的半数清除浓度（IC50）为42.32 μg/mL，其对照Vc标准品IC50为44.65 μg/mL；总黄酮对DPPH自由基的IC50为0.023 μg/mL，其对照Vc标准品IC50为0.011 μg/mL；花色苷作用于MDA-MB-231细胞24、48、72 h的IC50值分别为202.12、58.01、0.232 μg/mL;总黄酮作用于MDA-MB-231细胞24、48、72 h的IC50值分别为0.42、12.05、12.11 mg/mL；本研究为水提紫甘薯色素废渣的综合利用提供了科学依据。

Abstract:

Anthocyanins and total flavonoids present in pigment extraction residue obtained from Ipomoea batatas L. were evaluated for their biological activity. Inhibitory effects on Escherichia coli and Staphylococcus aureus were studied by filter paper diffusion, antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging ratio, and growth inhibition of human breast cancer cell line was determined by MTT assay. The results showed that proliferation of E. coli, S. aureus, and human breast cancer cell line, MDA-MB-231 was significantly inhibited, while significant DPPH radical-scavenging activity was also observed. The minimal inhibitory concentration (MIC) of anthocyanins and total flavonoids for E. coli and S. aureus was 400 and 4.0 mg/mL, respectively. Additionally, the values of half-maximal inhibitory concentration (IC50) and standard control (Vc) for DPPH radicals were 42.32 and 44.65 μg/mL for anthocyanins, and 0.023 and 0.011 μg/mL for flavonoids, respectively. The IC50 for MDA-MB-231 within 24, 48, and 72 h were 202.12, 58.01, and 0.232 μg/mL for anthocyanins, and 0.42, 12.05 and 12.11 mg/mL for flavonoids, respectively. These results demonstrate potential for value-added utilization of the residue after pigment extraction of Ipomoea batatas L.