基于高通量测序的浓香型白酒窖池细菌群落结构分析

High-throughput Sequencing Reveals Bacterial Structure in the Mud Pits of Heavy-fragrance Baijiu

摘要:

本试验利用高通量测序技术研究浓香型白酒窖池窖泥微生物群落结构，建立了一套完整的窖泥微生物群落结构研究方法和相关数据分析方法，同时得到了川北地区不同窖龄的窖泥微生物群落结构。通过Roche GS junior高通量测序平台对川北地区不同窖龄窖泥微生物群落结构进行分析后发现：6口窖池的窖泥主要细菌类群为Firmicutes(厚壁菌门)占79%，Actinobacteria (放线菌门)占4.9%、Bacteroidetes（拟杆菌门）占4.1%、Lentisphaerae (黏胶球形菌门)占3.5%、Synergistetes(互养菌门)占2.5%，其它占6%；其中Clostridiales Family XI. Incertae Sedis随着窖龄的增加呈现减少的趋势，Actinobacteria 类群和Synergistetes类群随着窖龄的增加呈现增加的趋势，而Haloplasmataceae类群和Clostridiaceae类群则只在30年窖龄的窖池中有检测出，Syntrophomonadaceae类群在5年窖龄的窖池中均有检出，Lactobacillaceae类群和Porphyromonadaceae类群则只在30年窖龄的一口窖池中是优势细菌类群。

Abstract:

High-throughput sequencing was used to study the composition of the microbial community within the mud pits of differently-aged, heavy-fragrance Baijiu cellars in northern using the Roche GS Junior high-throughput sequencing platform. The results showed that the main bacterial taxa in the six mud pits tested included 79% Firmicutes, 4.9% Actinobacteria, 4.1% Bacteroidetes, 3.5% Lentisphaerae, 2.5% Synergistetes, and 6% others. With increasing age of the cellar, the Clostridiales Family XI. Incertae Sedis showed a decreasing trend, while the Actinobacteria and Synergistetes taxa showed an increasing trend. The Haloplasmataceae and Clostridiaceae taxa were detected only in the mud pits of a 30-year-old cellar, while the Syntrophomonadaceae taxon was detected in the mud pits of all 5-year-old cellars. The Lactobacillaceae and Porphyromonadaceae taxa were the dominant bacterial groups in only one 30-year-old cellar mud pit.