荞麦粉对高脂膳食大鼠脂代谢的影响

Effect of Buckwheat on Lipid Metabolism in High-fat Diet-fed Rats

摘要:

本文研究了荞麦粉（buckwheat，BW）对高脂膳食大鼠脂代谢的影响。将50只成年Wistar雄性大鼠随机分为基础对照组、高脂模型组、荞麦粉低、中、高剂量组（1、5、10 g/kg?d），每天喂食一次，饲喂30 d后腹主动脉取血，取出肝脏、肾周及睾丸附近脂肪，分别测定血清和肝脏中的总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的水平，并观察肝脏病理切片。结果表明，与高脂膳食组相比，荞麦剂量组大鼠体重和食物摄入量都明显减少，肝重和脂肪重及其指数也明显降低，血清和肝脏中TC、TG、LDL-C的浓度降低，HDL-C的浓度升高。肝脏光镜结构表明，荞麦剂量组的肝脏脂肪变性均减轻，其中荞麦粉高剂量作用最为明显。说明荞麦粉干预高脂膳食，可起到降血脂、改善肝脏脂代谢、保护肝脏的作用，同时能够减少相关疾病的发生。

Abstract:

The aim of this study was to elucidate the effect of buckwheat (BW) on lipid metabolism in high-fat diet-fed rats. Fifty male rats were randomly divided into the following groups: control diet, high-fat diet (HFD), low-dose BW (1 g/kg?d), medium dose BW (5 g/kg?d), and high dose BW (10 g/kg?d). The rats were fed once a day for 30 days. Blood samples were subsequently collected from the posterior abdominal aorta, and the liver, in addition, perirenal fat, and epididymal fat were collected. The levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) in the serum and liver were measured, and pathological sections of the liver tissues were observed. The results showed that compared to the HFD group, the weight and food intake of all the BW groups were significantly lower, the weights and indexes of the liver and fat tissues were also significantly reduced, the concentration of TC, TG, and LDL-C in the serum and liver were decreased, and the concentration of HDL-C was increased. Meanwhile, the liver structure observed under the light microscope showed that hepatic steatosis in all BW groups was alleviated, with the most significant effect observed in the high-dose BW group. These results demonstrate that a BW intervention on a HFD can have a hypolipidemic effect, improve liver lipid metabolism, protect the liver, and reduce the incidence of related diseases.