不同钙补充剂对体外胃酸消耗及大鼠胃液分泌的影响

Effect of Different Calcium Supplements on in vitro Acid Consumption and Gastric Acid Secretion in Rats

摘要:

比较柠檬酸苹果酸钙、碳酸钙、牦牛骨粉、L-天门冬氨酸钙这四种钙补充剂溶解后对胃酸的消耗情况，及对大鼠胃液分泌的影响，为消费者选择合适的钙剂提供参考。通过体外模拟人工胃环境，测定四种钙剂在人工胃液中释放钙离子所消耗的胃酸量；使用大鼠幽门结扎法建立大鼠胃液分泌模型，观察不同钙剂对大鼠胃液量、胃酸酸度及胃酸分泌量的影响。实验表明柠檬酸苹果酸钙溶解后使人工胃液中氢离子含量显著增加（P<0.01），碳酸钙、牦牛骨粉和L-天门冬氨酸钙溶解后则使人工胃液中氢离子含量显著降低（P<0.05）；大鼠体内实验中，柠檬酸苹果酸钙组和牦牛骨粉组大鼠的胃液量、胃酸酸度及胃酸分泌量均显著减少（P<0.01），L-天门冬氨酸钙组大鼠的胃液量、胃酸酸度及胃酸分泌量均显著增多（P<0.01），碳酸钙组大鼠的胃液量、胃酸酸度、胃酸分泌量与正常对照组大鼠相比无显著性差异（P>0.05）。

Abstract:

Four calcium supplements including calcium citrate malate (CCM), calcium carbonate, yak bone meal, and calcium L-aspartate were administered to rats to compare their effect on gastric secretion as well as gastric acid consumption capacity in rats. The objective of this study was to identify appropriate calcium supplements for human consumption. Using a simulated gastric environment in vitro, the quantity of gastric acid consumed to quench the calcium ions released by the calcium supplements in artificial gastric juice was measured. The effects of calcium supplements on quantity, pH, and secretion of gastric juice were established using the pylorus-ligation rat model. The results showed that the hydrogen ion content increased significantly (p < 0.01) in artificial gastric juice after dissolving CCM, while it decreased significantly (p < 0.05) when the other three calcium supplements were dissolved. In vivo tests in rats showed that, as compared to the control group, the volume, acidity, and secretion of gastric acid decreased significantly (p < 0.01) in the CCM and bone meal groups, increased significantly in the calcium L-aspartate group, and did not show significant difference (p > 0.05) in the calcium carbonate group.