观察桑椹和桑椹酒多酚提取物对棕色脂肪细胞BAT-cMyc增殖的影响，探讨桑椹和桑椹酒多酚提取物对BAT-cMyc细胞棕色脂肪分化的效果。用不同浓度的桑椹和桑椹酒多酚提取物干预BAT-cMyc细胞48 h。采用MTT比色法检测桑椹和桑椹酒多酚提取物对BAT-cMyc细胞的生长抑制作用，计算IC50，并实时荧光定量法测定细胞增殖相关基因Ki67及PCNA相对表达量。再通过桑椹和桑椹酒多酚提取物处理BAT-cMyc细胞，直至分化第6 d，收集细胞。采用油红O染色法和实时荧光定量法对脂肪细胞分化效率进行分析。结果发现，桑椹和桑椹酒多酚提取物对BAT-cMyc细胞生长抑制的IC50浓度分别为186.37 μg/mL和158.78 μg/mL，且随着提取物浓度的升高对BAT-cMyc细胞的抑制增强。在浓度低于10 μg/mL时，桑椹和桑椹酒多酚提取物对BAT-cMyc细胞的增殖无影响，却可以显著增加BAT-cMyc的脂肪细胞的分化和上调棕色脂肪细胞分化相关基因，如AP2和C/EBPδ等的表达。

The effects of mulberry polyphenol extract (MPE) and mulberry wine polyphenol extract (MWPE) on the proliferation and differentiation of brown adipose tissue (BAT)-cMyc cells were investigated in this study. After treatment of BAT-cMyc cells with different concentrations of MPE and MWPE for 48 hours, the cells were collected, the growth inhibition effect of MPE and MWPE on BAT-cMyc cells was determined by MTT assay, and the IC50 values were calculated. The relative expressions of genes associated with cell proliferation, such as Ki67 and proliferating cell nuclear antigen (PCNA), were detected by real-time polymerase chain reaction (PCR). BAT-cMyc cells were then treated with MPE and MWPE until the sixth day of differentiation. Cells were collected and the efficiency of adipocyte differentiation was measured by Oil Red O staining and real-time PCR. The results showed that the IC50 value of BAT-cMyc cells was 186.37 μg/mL for MPE and 158.78 μg /mL for MWPE. Furthermore, growth of BAT-cMyc cells was inhibited in a dose-dependent manner. MPE and MWPE had no effect on BAT-cMyc cell proliferation, but could significantly increase the efficiency of the differentiation of BAT-cMyc cells into adipocytes and upregulate the expression levels of brown adipocyte differentiation-related genes, such as AP2 and C/EBPδ, during brown adipogenesis.

公益性行业(农业部)科研专项（201303073）；国家“十二五”科技支撑计划（2012BAD31B07）