桑根氧化白藜芦醇的分离、稳态化包埋及其酪氨酸酶活性抑制作用研究

The Separation of Oxyresveratrol from Mulberry Root and Its Effect on Steady-state Embedding and Inhibition of Tyrosinase Activity

摘要:

氧化白藜芦醇是一种重要的活性天然产物，是反式二苯乙烯类天然产物白藜芦醇(Resveratrol)的2'-位羟基化衍生物，具有美白、抗癌、保肝、抗氧化、抗衰老、抗炎等作用。本研究在建立了精确检测氧化白藜芦醇高效液相色谱（HPLC）方法基础上，以桑根为原料，研究了氧化白藜芦醇的提取分离、稳态化包埋及对酪氨酸酶活性的抑制作用。结果表明：以80%乙醇为提取溶剂，在提取温度70 ℃，时间1.5 h，液料比4:1，提取次数3次条件下，氧化白藜芦醇提取得率为94.32%；在固形物含量为40%，进风温度为190 ℃，雾化盘转速为16000 r/min，进料速度为1.78 L/h条件下，氧化白藜芦醇微胶囊稳态化包埋率为98.91%；氧化白藜芦醇标准品、氧化白藜芦醇微胶囊样品具有对酪氨酸酶活性抑制作用，且抑制强弱呈剂量依赖型。

Abstract:

Oxyresveratrol is a 2'-hydroxyl derivative of resveratrol (a trans-stilbene); it is an important natural bioactive product with whitening, anticancer, antioxidant, hepatoprotective, anti-inflammatory, and anti-aging functions. In this study, high-performance liquid chromatography (HPLC) was used to detect oxyresveratrol in mulberry root, and its extraction, separation, steady-state embedding, and inhibitory effects on tyrosinase activity were examined. The extraction rate of oxyresveratrol was 94.32% for the following conditions: 80% ethanol as the extraction solvent, an extraction temperature of 70 ℃, an extraction duration of 1.5 h, a liquid to solid ratio of 4:1, and independent extraction of three times. The steady-state embedding rate of oxyresveratrol was 98.91% for the following conditions: a solid content of 40%, an inlet temperature of 190 ℃, an atomizing disk speed of 16,000 r/min, and a feed speed of 1.78 L/h. The oxyresveratrol standard and oxyresveratrol microcapsule samples both had inhibitory effects on tyrosinase activity, and the strength of inhibition was dose-dependent.