中国主要水果抑制肝癌HepG2细胞和结肠腺癌Caco-2细胞增殖活性评价

Evaluation of the Anti-proliferative Activities of Major Fruits in China on Human Hepatic Carcinoma (HepG2) and Colon Adenocarcinoma (Caco-2) Cells

摘要:

分别采用Folin-Ciocalteu法测定了水果提取物的总酚含量，采用亚甲基蓝法测定了其抗人肝癌细胞HepG2和人结肠腺癌细胞Caco-2增殖的活性，分析了总酚含量与抗HepG2和Caco-2细胞增殖活性之间的相关性。结果显示，25种水果中李子的总酚含量最高（1686.08±96.94 ?mol GAE/100 鲜果），西瓜（83.54±2.10 ?mol GAE/100 g鲜果）和哈密瓜（79.35±0.76 ?mol GAE/100 g鲜果）的总酚含量最低；在可测出抗增殖EC50值的水果中，李子（18.99±0.06 mg/mL）和番石榴（20.94±0.09 mg/mL）抗HepG2细胞增殖的活性最强，梨的活性最弱（389.63±10.82 mg/mL）。李子抗Caco-2细胞增殖的活性最强（8.73±0.11 mg/mL），火龙果的活性最弱（388.07±7.04 mg/mL）。水果的抗HepG2和Caco-2细胞增殖活性与其总酚含量相关性显著（R2=0.4147，p<0.01；R2=0.4071，p<0.05），该相关性表明水果中的多酚具有良好的抗肿瘤细胞增值的活性。

Abstract:

The total phenolic content and the anti-proliferative activities of the phenolic extracts against HepG2 and Caco-2 cells were determined by the Folin-Ciocalteu method and the methylene blue assay, respectively; the correlation between the total phenolic content and the anti-proliferative activity of the extracts was analyzed. The results of these analyses revealed that plum (Prunus salicina Lindl.) had the highest total phenolic content (1686.08 ± 96.94 ?mol gallic acid equivalent (GAE)/100 g of fresh fruit), whereas watermelon (Citrullus lanatus) (83.54 ± 2.10 ?mol GAE/100 g of fresh fruit) and hami melon (Cucumis melo var. saccharinus) (79.35 ± 0.76 mol GAE/100g of fresh fruit) had the lowest phenolic content among the 25 tested species. Among the fruit species with quantifiable anti-proliferative activities (half maximal effective concentration (EC50)), plum and guava (Psidium guajava Linn.) showed the strongest anti-proliferative activities against HepG2 cells (EC50 values: 18.99 ± 0.06 and 20.94 ± 0.09 mg/mL, respectively), whereas pears (Pyrus spp.) showed the weakest anti-proliferative activity against these cells (EC50 value: 389.63 ± 10.82 mg/mL). Plum also demonstrated the strongest anti-proliferative activity against Caco-2 cells (EC50 value: 8.73 ± 0.11 mg/mL), while the activity of pitava (Hylocereus undulatus Britt.) was observed to be the weakest (EC50 value: 388.07 ± 7.04 mg/mL). The anti-proliferative activities of fruits on HepG2 and Caco-2 cells were significantly correlated to their total phenolic content (R2 = 0.4147, p < 0.01; R2 = 0.4071, p < 0.05, respectively). This correlation indicates the high anti-proliferative effect of fruit polyphenols on cancer cells.