联苯三唑醇分子印迹电化学传感器的制备及识别性能研究

Preparation and Recognition Properties of a Bitertanol Electrochemical Sensor Based on Molecular Imprinting Technology

摘要:

以联苯三唑醇为模板分子，通过电聚合邻苯二胺，在玻碳电极表面制备了对模板分子具有特异识别的分子印迹电化学传感器。实验选用铁氰化钾(K3[Fe(CN)6])作为电活性探针，采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了该传感器的电化学特性，并且优化了制备条件，研究了印迹传感器对模板分子及其结构类似物的选择性响应。结果表明：在最佳聚合条件下，pH=7.2，模板分子:功能单体=1:4(c/c)，聚合圈数15圈，洗脱时间为13 min，洗脱剂：0.5 mol/L硫酸:乙腈=1:9 (V/V)，连续洗脱10次之后，相对标准偏差为5.2%，且传感器性能无衰减现象。该分子印迹电化学传感器灵敏度高，抗干扰能力强，对联苯三唑醇及其结构类似物具有良好的选择性响应，可以对结构类似物分别进行测定，为快速检测食品中联苯三唑醇奠定了基础。

Abstract:

A sensitive, molecularly imprinted electrochemical sensor using bitertanol as the template molecule for the detection of bitertanol in foods was prepared by the electropolymerization of o-phenylenediamine on a glassy carbon electrode. The electrochemical properties of the molecularly imprinted sensor were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using K3[Fe(CN)6] as the probe; the preparation conditions were optimized, and the selective responses of the molecularly imprinted sensor towards the template molecule and compounds with similar structures were investigated. The results showed that the optimal conditions to prepare the sensor were as follows: pH of 7.2; ratio of template molecule to functional monomer = 1:4; 15 polymerization circles; elution time of 13 min; 0.5 mol/L sulfuric acid : acetonitrile = 1:9 (V/V) as eluent. The relative standard deviation was 5.2% and the sensor performance was not attenuated after continuous elution of the electrode 10 times. The sensor had a high sensitivity, strong anti-interference ability, and good selective response towards bitertanol and compounds with similar structures. Using this sensor, compounds with similar structures could be determined separately, laying the foundation for the rapid detection of bitertanol in foods.