紫外线诱导海参体壁基因表达的研究

UV-Induced Gene Expressions in Sea Cucumber (Stichopus japonicus) Body Wall

摘要:

本论文对紫外线（58 μW/cm2，30 min）诱导的海参体壁自溶进行了基因表达的研究。采用TRIzol法提取海参体壁中的总RNA，以细胞色素B (CytB)为内标，采用反转录聚合酶链式反应(RT-PCR)对组织蛋白酶C (CC)、纤维蛋白原A (FGL)、衰老相关蛋白(SAP)和主要卵黄蛋白2 (MYP2)、组织蛋白酶L (CL)、钙网蛋白(Calreticulin)、基质金属蛋白酶14 (MMP14)、乙酰胆碱酯酶(AchE)等29种分子进行基因扩增。结果显示，紫外线照射30 min后，海参体壁组织蛋白酶C、纤维蛋白原A、衰老相关蛋白和主要卵黄蛋白2的基因表达水平显著上调（p<0.05），上调水平分别较对照组提高了71.4±44.8%、27.1±18.4%、43.7±21.6%和165.5±122.7%，而组织蛋白酶L、钙网蛋白、基质金属蛋白酶14、乙酰胆碱酯酶等基因表达没有显著变化。这些结果表明组织蛋白酶C、纤维蛋白原A、衰老相关蛋白和主要卵黄蛋白2可能参与海参体壁的自溶过程。

Abstract:

In this study, gene expressions for UV-induced (58 μW/cm2, 30 min) body wall autolysis of sea cucumber (Stichopus japonicus) were investigated. Total RNA was extracted using the TRIzol method from sea cucumber body wall. Twenty-nine genes, including cathepsin C (CC), fibrinogen-like protein A (FGL), senescence-associated protein (SAP), major yolk protein 2 (MYP2), cathepsin L (CL), calreticulin, matrix metalloproteinases14 (MMP14), and acetylcholinesterase (AChE), were amplified using reverse transcription-polymerase chain reaction with cytochrome B (CytB) as an internal control. The results indicated that the gene expressions of CC, FGL, SAP, and MYP2 were significantly enhanced by 71.4 ± 44.8%, 27.1 ± 18.4%, 43.7 ± 21.6%, and 165.5 ± 122.7%, respectively, after UV irradiation for 30 min (p < 0.05). However, the gene expressions of CL, calreticulin, MMP14, and AChE did not change significantly. These results suggest that CC, FGL, SAP, and MYP 2 may be involved in the autolysis of sea cucumber body wall.