麒麟菜多糖对H22肝癌移植瘤的抑制作用研究

Inhibitory Effect of Eucheuma gelatinae Polysaccharides on H22 Hepatoma Xenograft Cells

摘要:

以常温稀碱法提取的麒麟菜多糖（Eucheuma gelatinae polysaccharides，EGP）饲喂H22荷瘤小鼠，探讨其对小鼠移植性实体瘤的抑制作用。实验考察了肿瘤抑制率和免疫器官指数，分析荷瘤小鼠的脾淋巴细胞（T淋巴细胞与B淋巴细胞）增殖能力、脾NK细胞杀伤活性和腹腔巨噬细胞吞噬能力等免疫指标，并对外周血淋巴细胞亚群进行流式（FCM）检测。研究结果表明，以麒麟菜多糖灌胃荷瘤小鼠能够显著抑制小鼠体内肿瘤的生长，至实验期结束时，麒麟菜多糖低剂量组[300 mg/(kg?d)]及高剂量组[600 mg/(kg?d)]的抑瘤率分别达到31.17%及59.67%。与模型组相比，麒麟菜多糖还能够显著增强荷瘤小鼠脾淋巴细胞的增殖能力和NK细胞的杀伤活性，并显著提高腹腔巨噬细胞的吞噬活性。此外，麒麟菜多糖高剂量组能显著提高荷瘤小鼠CD3+和CD8+细胞比例(p<0.05)。麒麟菜多糖能够改善荷瘤小鼠机体的免疫水平，具有显著的抗肿瘤作用。

Abstract:

In this study, the antitumor effect of Eucheuma gelatinae polysaccharides (EGP) on H22 transplanted solid tumor cells was investigated. Mice were orally administered EGP extracted by alkali treatment at room temperature. The tumor inhibition rate, immune organ index, proliferation ability of splenocytes (T and B lymphocytes), splenic NK cell killing activity, and phagocytosis by peritoneal macrophages were measured. Moreover, peripheral blood lymphocyte subsets were investigated by flow cytometry. The results showed that EGP significantly inhibited H22 hepatoma cell growth in mice, which were transplanted with tumor and gavaged with EGP. The tumor inhibition rate by EGP was 31.17% in the low-dose group [300 mg/(kg?d)] and 59.67% in the high-dose group [600 mg/(kg?d)] at the end of the experimental period. Compared with the model group, EGP significantly promoted splenocyte proliferation, killing activity of NK cells, and macrophage phagocytosis. In addition, the ratio of CD3+ to CD8+ T cells in the tumor-bearing mice was significantly increased (p < 0.05) in the high-dose EGP group. The results suggested that EGP improved immunity of tumor-bearing mice and exerted a remarkable anti-tumor activity.