豆渣石油醚萃取物的FT-IR和GC/MS分析

Analysis of Petroleum Ether Extract from Soybean Dreg by FT-IR and GC/MS

摘要:

为了解豆渣中小分子物质的赋存形态，本研究用石油醚作溶剂在索式萃取器中对豆渣进行了萃取，并采用傅里叶转换红外光谱仪（FT-IR）和气相色谱/质谱联用仪（GC/MS）对所得石油醚萃取物进行分析，同时用FTIR对原料豆渣和萃余残渣也进行了分析。结果表明：实验连续萃取5 h，所得石油醚萃取物为淡黄色油状物，萃取率为10.7%；原料和萃余残渣的红外谱图峰形相近，但各吸收峰的吸收强度有所不同，而石油醚萃取物与原料和萃余残渣的红外谱图有明显的差别；石油醚萃取物中共检测到35种GC/MS可识别小分子有机化合物，主要分为烷烃、烯烃、芳香烃、醛、酸、酯及其它类物质7种类别；其中，酯类化合物相对含量为最多，高达61.79%。该研究在开发豆渣的高附加值利用方面具有重要的基础理论意义。

Abstract:

In order to understand the chemical speciation of small molecules in soybean dreg, they were was extracted with petroleum ether using a Soxhlet extractor. The petroleum ether extract was analyzed by Fourier transform infrared (FT-IR) spectroscopy and gas chromatography/mass spectrometry (GC/MS). The raw bean dreg and the residue from the extraction were also analyzed by FT-IR spectroscopy. A pale yellow oil was obtained from the petroleum ether extract after 5-hour continuous extraction, with an extraction yield of 10.7%. FT-IR band shapes were similar for the raw material and the residue from extraction, but with different absorption intensities, while obvious differences in the FT-IR spectra were found between the petroleum ether extract and raw bean dreg/the residue from the extraction. In total, 35 compounds were identified from the petroleum ether extract, which can be classified into seven groups: alkanes, alkenes, arenes, aldehydes, acids, esters, and other species. Among them, the esters were the most abundant compounds, with a relative content of 61.79%. This study was important for the basic theory on developing the value-added utilization of soybean dreg.