Abstract:In this study, the effects of two extraction methods on in vivo antioxidant activity in a mouse model for aging were evaluated. First, cellulase-assisted and ultrasound-assisted extractions were used to obtain grape seed oil, which was refined and administered by gavage to the aging mice (D-galactose-induced). The contents of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione and peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in the cerebrum, heart, liver, and serum of the mice were measured. The results showed that the contents of T-AOC, SOD, and GSH-Px in the cerebrum, heart, liver, and serum of the model group all showed different degrees of reduction and the MDA content increased to different degrees, indicating that the mouse model for aging was successfully established. Compared to the model group, each dose group of two kinds of extracted grape seed oil could both enhance the activities of T-AOC, SOD, and GSH-Px and reduce MDA content in the serum and cerebrum, heart, and liver tissues to various degrees. The grape seed oil obtained using cellulase-assisted extraction was slightly superior to that obtained using ultrasound-assisted extraction in terms of enhancing T-AOC and GSH-Px activities. These findings demonstrated that grape seed oil could obviously improve antioxidant activity in the mice; the in vivo antioxidant activity of grape seed oil obtained using cellulase-assisted extraction was slightly higher than that of the grape seed oil obtained using ultrasound-assisted extraction.
