酵母三股螺旋结构β-葡聚糖测定方法研究

Method to Determine the Triple-Helical β-glucan Content in Yeast

摘要:

建立了刚果红法快速检测酵母三股螺旋结构活性β-葡聚糖的方法，检测体系中酵母三股螺旋结构β-葡聚糖的助溶剂二甲基亚砜终浓度为2%，最大吸收波长为523 nm，β-葡聚糖的质量（Y）与ΔOD523值（x）的回归方程为Y=923.0x-5.641，线性系数R2=0.996，β-葡聚糖的质量在35~199 μg与相应的ΔOD有良好的线性关系。刚果红法测得酵母三股螺旋结构β-葡聚糖的质量的精密度高，其相对标准偏差为2.21%；该方法测得酵母细胞壁样品中三股螺旋结构β-葡聚糖的含量为18.33%，回收率在97.68~103.90%之间，平均回收率为99.60%，回收率的相对标准偏差为2.32%。说明刚果红法测定三股螺旋结构β-葡聚糖的回收率高，适用于酵母三股螺旋结构活性β-葡聚糖的测定，是一种快速、简便且高效的适用于工业生产过程检测的测定方法。

Abstract:

A quick method to determine the triple-helical β-glucan content in yeast using Congo red was established. The final concentration of dimethyl sulfoxide, which was the cosolvent for the yeast triple-helical β-glucan in the measuring system, was 2%, and the maximum absorption wavelength of the Cogon red system was 523 nm. The regression equation for the β-glucan mass (Y) and ΔOD523 value (x) was Y = 923.0x - 5.641, with a coefficient of determination (R2) of 0.996. The β-glucan mass and ΔOD demonstrated a strong linear relationship in the mass range of 35~199 μg of β-glucan. High accuracy was achieved using the Congo red method to measure yeast triple-helical β-glucan, with a relative standard deviation (RSD) of 2.21%. Using this method, the content of triple-helical β-glucan in yeast cell wall samples was 18.33%. The recovery rate varied from 97.68% to 103.90% (average, 99.60%), and the RSD of the recovery rate was 2.32%. The results indicated that the Congo red method used to determine the triple-helical β-glucan content had a high recovery rate and was suitable to measure yeast triple-helical β-glucan. It was a rapid, simple, and efficient determination method that could meet the demands of the industrial production process.