蛋白水解肽-Fe2+配合物对克氏原螯虾生长及非特异性免疫的影响

Effect of Protein Hydrolysate-Fe2+ Complex on the Growth Performance and Non-specific Immune Parameters of Procambarus clarkii

摘要:

在饲料中添加蛋白水解肽-Fe2+配合物（TPH-Fe2+），探讨其对克氏原螯虾生长及非特异性免疫的影响。生长性能结果表明，饲喂60 d后，400~1000 mg/kg TPH-Fe2+添加组与空白组相比，螯虾成活率、增重率、特定增长率及出肉率分别提高9.94~10.10%、8.55~8.72%、0.24~0.29%/d及2.39~3.05%（P<0.05）；添加800、1000 mg/kg时，显著提高螯虾粗蛋白及粗脂肪含量。免疫结果表明，饲喂30 d，200、400 mg/kg TPH-Fe2+对螯虾血清和肝胰腺SOD、PO、LSZ和ACP活力，与空白组相比较无显著性差异（P>0.05）；800、1000 mg/kg TPH-Fe2+显著提高螯虾体内SOD、LSZ和ACP酶活力（P<0.05）。饲喂60 d，400、600 mg/kg TPH-Fe2+对螯虾血清和肝胰腺中SOD、LSZ活力，表现出显著性提高作用（P<0.05），而对PO、ACP活力无显著性影响（P>0.05）；添加达800、1000 mg/kg时，显著提高螯虾体内SOD、PO、LSZ和ACP酶活力（P<0.05）。综合考虑螯虾生长性能及非特异性免疫酶活性，在饲料中添加TPH-Fe2+800~1000 mg/kg为宜。

Abstract:

The purpose of this study was to investigate the effects of addition of protein hydrolysate-Fe2+ complex (TPH-Fe2+) to feed on the growth performance and non-specific immune parameters of Procambarus clarkii. After 60 days of consumption of the basal feed supplemented with 400~1000 mg/kg TPH-Fe2+, the survival rate, weight, specific growth rate, and meat yield were increased by 9.94%~10.10%, 8.55%~8.72%, 0.24%~0.29%/d and 2.39%~3.05%, respectively (P < 0.05), compared to the control group. When 800 and 1000 mg/kg TPH-Fe2+ were added to the feed, the crude protein and fat contents of P. clarkii increased significantly. After 30 days of consumption of the basal feed supplemented with 200 and 400 mg/kg TPH-Fe2+, superoxide dismutase (SOD), phenoloxidase (PO), lysozyme (LSZ), and acid phosphatase (ACP) activities in the serum and hepatopancreas did not differ significantly from those in the control group (P > 0.05). However, feed supplementation with 800 and 1000 mg/kg TPH-Fe2+ significantly increased the SOD, LSZ, and ACP activities (P < 0.05). After 60 days of feeding with 400 and 600 mg/kg TPH-Fe2+, the activities of SOD and LSZ were significantly enhanced, but the activities of PO and ACP were not significantly affected. When 800 and 1000 mg/kg TPH-Fe2+ were added to the feed, the SOD, PO, LSZ, and ACP activities were all significantly increased (P < 0.05). In conclusion, the addition of 800~1000 mg/kg TPH-Fe2+ to the basal feed could improve the growth performance of and activities of non-specific immune enzymes in P. clarkii .