紫外光照射和γ射线辐照对大豆中黄曲霉毒素B1降解的比较研究

Degradation of Aflatoxin B1 in Soybean by Ultraviolet and γ Irradiation

摘要:

以新鲜大豆为原料，通过标准液添加获得黄曲霉毒素B1（AFB1）含量分别为0.1、0.5和1.0 mg/kg的染毒大豆，考察了紫外光照射、γ射线辐照及其联合技术对大豆中AFB1的降解效果。结果表明，利用紫外光照射降解大豆中AFB1时，紫外光照射强度对大豆中AFB1的降解具有显著影响（P<0.05），而大豆中水分含量和AFB1含量对AFB1降解率的影响并不显著（P>0.05），紫外光照射前10 min内，大豆中AFB1的降解率随光照时间的增加而增加，但是当照射时间超过10 min后，紫外光照射对大豆中AFB1降解率的影响并不显著（P>0.05）。利用γ射线辐照降解大豆中AFB1时，大豆中AFB1降解率随γ射线辐照剂量和大豆水分含量的增加而增加，随大豆中AFB1含量的增加而降低，γ射线辐照剂量、水分含量和AFB1含量对大豆中AFB1的降解均具有显著影响（P<0.05）。同时，研究还发现紫外光照射和γ射线辐照对大豆中AFB1的降解具有耦合增效作用。

Abstract:

The aim of this research was to study the effect of ultraviolet irradiation, γ irradiation, and a combination of the two on the degradation of aflatoxin B1 (AFB1) in soybean. In this work, fresh soybean was used as the raw material, and contaminated soybeans that contained 0.1, 0.5, and 1.0 mg AFB1/kg soybean were obtained by adding AFB1 to soybean. The ultraviolet irradiation intensity had a significant influence on the degradation of AFB1 (P<0.05), whereas the moisture content and AFB1 content had no significant effects on the degradation (P>0.05). The degradation of AFB1 increased with increasing ultraviolet irradiation time up to 10 min, after which the degradation ratio remained stable. On the other hand, the degradation of AFB1 increased with increasing γ irradiation dose and moisture content, but decreased with increasing AFB1 content. The γ irradiation dose, moisture content, and AFB1 content had a significant influence on the degradation of AFB1 (P<0.05). A synergistic effect between ultraviolet irradiation and γ irradiation was also observed.