为研究春季浒苔与冬季浒苔的挥发性物质种类及成分的差异,本文通过电子鼻和顶空固相微萃取与气质联用仪（HS-SPME-GC-MS）联合使用检测分析冬季与春季浒苔挥发性物质差异。电子鼻各传感器采集数值经过t检验分析结果得出春冬两季浒苔的挥发性物质种类及成分有明显差异；HS-SPME-GC-MS分析检测春季、冬季浒苔的挥发性化合物种类数分别为68、82种，并且前者挥发性化合物中以烃类和醛类物质为主，后者挥发性化合物中以醛类和酮类为主。两者挥发性物质相对含量差异主要体现在醇类、酮类、酸类、烃类以及杂环类物质。同时在杂环类物质中，冬季浒苔检测出苦味物质甲基吡嗪，相对含量为0.13%；春季浒苔未检测出。由此得出浒苔在不同季节下其挥发性物质种类及含量有明显不同。

In this study, the composition and spectrum of volatile compounds in Enteromorpha prolifera harvested from winter and spring, were analyzed using electronic nose (E-nose) and head space solid-phase micro extraction combined with gas chromatography mass spectrometry (HS-SPME-GC-MS). The data from E-nose sensors and t-test results showed significant differences in the composition and types of volatile compounds in E. prolifera between samples collected during the two seasons. HS-SPME-GC-MS analysis identified 68 and 82 different volatile compounds in E. prolifera harvested in spring and in winter, respectively. In addition, the main volatile compounds in the former were hydrocarbons and aldehydes, whereas those in the latter were aldehydes and ketones. The relative content of volatile compounds between samples contained in the two seasons differed mainly with respect to alcohols, ketones, acids, hydrocarbons, and heterocyclic compounds. At the same time, among the heterocyclic compounds, a bitter compound, methylpyrazine, was detected in E. prolifera harvested in winter, with a relative content of 0.13%, but it was not detected in samples harvested in spring. Therefore, significant differences were observed in the composition and the types of volatile compounds in E. prolifera harvested in different seasons.

浙江省重大科技专项重点社会发展项目(2009C03017-1)