陕西市售国产葡萄酒中多酚类物质的特征与抗氧化能力分析

Polyphenol Composition and Antioxidant Activity of Domestic Wines from Shaanxi Province

摘要:

以陕西市售国产葡萄酒为样本，通过评价其多酚成分与抗氧化能力，分析其产品质量与健康价值。采用Folin-Ciocalteu法测定总酚、三氯化铝法测定总类黄酮、DMACA法测定总黄烷-3-醇、pH示差法测定总花色苷含量；HPLC法测定单体酚；DPPH法测定抗氧化能力。结果显示，陕西市售国产葡萄酒多酚类物质含量较高，单体酚组成丰富，符合葡萄酒类型、产地与品种特征。但也有个别样品有外源添加多酚类物质。陕西市售国产葡萄酒中无半汁或掺假葡萄酒存在。并且，陕西市售国产葡萄酒抗氧化能力较高，具有一定的健康价值。与单纯依靠品酒师来评估葡萄酒质量，受限于专业水平与个人喜好相比，以多酚组成、抗氧化能力等参数来对葡萄酒质量进行评估是可行的，行之有效的一种方法。本研究结果有助于为消费者提供除价格外的新的参考。

Abstract:

The quality and health benefits of domestic wines sold in the cities of Shaanxi province was evaluated, in terms of polyphenol composition and antioxidant activity. We determined the content of total phenol by Folin-Ciocalteu method, total flavonoids by aluminum chloride method, total flavan-3-ols by p-dimethylaminocynnamaldehyde method, total anthocyanins by pH differential method, monomeric phenols by high-performance liquid chromatography, and antioxidant activity by 1-diphenyl-2-picrylhydrazyl method. The results showed that the sampled domestic wines from Shaanxi Province have a high content of polyphenolic compounds and rich monomeric phenols, which correlated with the designated type, origin, and varietal characteristics of the individual samples. However, few samples also contained exogenously added polyphenolic compounds. No adulterated wines or half-juice variants were found among the sampled domestic wines sold in these cities. In addition, high antioxidant activity was detected in the samples, implying health benefits on their consumption. Evaluation of wine quality by wine tasters is limited by the individual competence and personal preference, whereas the current method uses polyphenol composition and antioxidant activity to evaluate wine quality and therefore, is feasible and effective in comparison. The findings of this study could provide a new quality reference for consumers besides that indicated by the price.