裂褶菌多糖对小鼠免疫活性作用的研究

Immunological Effects of Schizophyllum commune Polysaccharides in Mice

摘要:

本文以裂褶菌多糖为研究对象，通过超声波辅助水提法提取裂褶菌多糖，对小鼠腹腔注射环磷酰胺（Cy）建立免疫低下模型，灌胃不同剂量的裂褶菌多糖，从非特异性免疫、细胞免疫和体液免疫三种途径来研究裂褶菌多糖的免疫活性作用。同时采用MTT法研究了裂褶菌多糖抑制体外小鼠巨噬细胞及结肠癌细胞增殖的作用。试验结果表明，裂褶菌多糖对免疫低下小鼠的胸腺、脾脏器官具有一定的恢复作用；对小鼠足趾注射绵羊红细胞致敏，足趾具有一定的肿胀度；通过测定小鼠血清中血清溶血素、IgG、IgA、IL-2，裂褶菌多糖的各剂量与免疫低下的模型组相比均有不同程度的提高；裂褶菌多糖可增强小鼠巨噬细胞的吞噬能力，当浓度为2000 μg/mL时，对结肠癌细胞增殖的抑制率可达到45.32%。

Abstract:

In this study, polysaccharide from Schizophyllum commune was obtained by ultrasound-assisted water extraction and an immunocompromised mouse model was established by intraperitoneal injection of cyclophosphamide. Immunological effects of different doses of S. commune polysaccharides administered to mice intragastrically were investigated in terms of non-specific immunity, cell-mediated immunity, and humoral immunity. The inhibitory effects of S. commune polysaccharides on the in vitro proliferation of mouse macrophages and colon carcinoma were evaluated by MTT assay. The results showed that S. commune polysaccharides had a certain recovery effect on the thymus and spleen as well as caused sensitization in mice injected with sheep red blood cells in their footpads, leading to swelling. In mice injected with different doses of S. commune polysaccharides, serum levels of hemolysin, IgG, IgA, and IL-2 increased to varying degrees as compared to the immunocompromised mice group. In addition, S. commune polysaccharides could increase phagocytosis by murine macrophages and at 2000 μg/mL concentration, showed up to 45.32% inhibition of colon carcinoma cells.