多不饱和脂肪酸对小鼠3T3-L1前脂肪细胞增殖和分化的影响

Effect of n-3/n-6 Polyunsaturated Fatty Acid on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

摘要:

本文利用MTS比色分析法、细胞形态学分析法及诱导分化法结合油红染色比色分析法研究了α-亚麻酸（alpha-linolenic acid，ALA）、亚油酸（linoleic acid，LA）对3T3-L1前脂肪细胞增殖及分化的影响。MTS结果显示，在一定浓度范围内ALA、LA均能呈剂量依赖关系和时间依赖关系的抑制3T3-L1前脂肪细胞的增殖，且ALA抑制增殖作用强于LA；细胞形态学分析结果显示，ALA、LA在高浓度时均显示出细胞毒性作用；倒置显微镜观察和油红染色检测结果显示，ALA、LA、ALA/LA混合脂肪酸（1:1，n/n）在12.5~200 μmol/L浓度范围内均能浓度依赖式的抑制3T3-L1前脂肪细胞分化，且ALA抑制分化作用强于LA，但当浓度>100 μmol/L时，ALA/LA混合脂肪酸（1:1，n/n）的抑制分化作用强于同浓度的ALA，当浓度为200 μmol/L时，比较ALA/LA摩尔比为1:4、1:2、1:1的混合脂肪酸抑制分化效果发现，比值为1:1的略强但相互之间效果差异不明显。

Abstract:

The effects of alpha-linolenic acid (ALA) and linoleic acid (LA) on the proliferation and differentiation of 3T3-L1 preadipocytes were investigated by MTS assay, cell morphology analysis and spectrometric method of induction differentiation combined with oil red O staining. MTS results showed that ALA and LA significantly inhibited proliferation of 3T3-L1 preadipocytes in a dose- and time-dependent manner within a certain concentration range, and ALA inhibited proliferation more strongly than LA. Cell morphology analysis showed that ALA and LA were cytotoxic to 3T3-L1 preadipocytes because of high concentration of the fatty acids. The inverted electron microscope observing and oil red staining and detection results showed that ALA, LA and ALA/LA mixed fatty acid (1:1, n/n) significantly inhibited differentiation of 3T3-L1 preadipocytes in a dose-dependent manner in the range of 12.5~200 μmol/L, and ALA inhibited differentiation more efficiently than LA, and the ALA/LA mixed fatty acid (1:1, n/n) showed the strongest inhibition effect on differentiation at the same concentration above 100 μmol/L. When the concentration was 200 μmol/L, the ALA/LA mixed fatty acid (1:1, n/n) showed slightly lower inhibition effect on the differentiation of 3T3-L1 preadipocytes than the ALA/LA mixed fatty acid (1:2, n/n) and the ALA/LA mixed fatty acid (1:4, n/n).