低分子量姬松茸多糖对胃癌BGC823细胞生长和侵袭的影响

Influence of the Low-molecule-weight Polysaccharides of Agaricus blazei Murrill on Proliferation and Invasion in Gastric Cancer BGC823 Cells

摘要:

通过观察低分子量姬松茸多糖(LABP)对胃癌细胞系BGC823增殖、侵袭的影响及作用过程中相关分子蛋白水平表达的变化，探讨其对BGC823细胞影响的机制。运用WST-1和Transwell实验检测LABP对胃癌BGC823细胞生长和侵袭的影响；采用Western blot实验检测相关分子蛋白质水平表达的变化。与空白对照组相比，LABP组细胞数量明显减少（P<0.05），且呈浓度依赖性，当LABP浓度为1×10-4 g/mL时，对BGC823细胞的抑制率分别为33.4%、69.8%。LABP组穿透过膜细胞较空白对照组明显减少， 细胞侵袭能力受到抑制；LABP组中Caspase 3蛋白表达上升（P<0.05）；MMP 9蛋白表达明显降低（P<0.01），而E-cadherion无显著性差异（P>0.05）。结果表明低分子量姬松茸多糖（LABP）对胃癌BGC823细胞的生长和侵袭具有抑制作用。LABP可能通过上调Caspase 3蛋白表达，下调MMP 9蛋白表达，抑制胃癌BGC823细胞的生长。

Abstract:

The effect of polysaccharides with low molecule weight from Agaricus blazei Murrill (LABP) on proliferation and invasion in gastric cancer cell line BGC823 was observed by WST-1 and Transwell assay, and the expression changes of some related molecules in proteinic level were determined by Western blot, to explore the mechanism of LABP affected on BGC823 cells. Compared with the control group, LABP group cells were significantly declined (P<0.05) in a dose-dependent manner. BGC823 cell inhibition rates by LABP were 33.4% and 69.8%, respectively on LABP concentration of 1 × 10-4g/mL. The cells permeated the membrane of LABP groups were obviously less and the protein expression of Caspase 3 of LABP group was dramatic increased (P<0.05). The expression of MMP 9 was dramatic down-regulated (P<0.05), while E-cadherion had no significant difference (P>0.05). Thus, low-molecule polysaccharide of Agaricus blazei Murrill (LABP) had a dramatic inhibiting effect on growth and invasion in gastric cancer BGC823 cells. LABP inhibited the gastric cancer BGC823 cells growth, possibly by up-regulating the protein expression of Caspase 3 and down-regulating MMP 9.