Polycyclic aromatic hydrocarbons (PAHs) in seafood samples were ascribed to water pollution, while benzo(a)pyrene was recognized as an indicator of PAHs. A simple and rapid analytical method was developed by combining microwave-assisted pretreatment with second-derivative constant-energy synchronous fluorescence scanning technique for the determination of the benzo(a)pyrene content in seafood samples. The samples were firstly processed with microwave pretreatment, and the extractions were vaporized and redissolved into dichloromethane for further detection. According to the specific spectral properties of seafood samples, peak-to-derivative baseline measurement was used to read the fluorescence data, and standard addition method was applied to the quantitative analysis of seafood samples. The whole spectroscopic scan cost only 1 min. The recoveries ranged from 80.5% to 118.2% for seafood samples and the detection limit was 0.10 μg/kg with linear range from 0.34 to 250 μg/kg. The results obtained with this method were in consistence with those of HPLC-FL method. The satisfactory results acquired by using this method to analyze a certified reference material with a relative error of 7.1% from the certified benzo(a)pyrene concentration.
