基于ESR和细胞培养体系的海参肠自溶寡肽抗氧化活性研究

Analysis of Antioxidant Activities of Oligopeptides from Sea Cucumber Guts Based on ESR and Cell Culture System

摘要:

本文在前期工作基础上，研究了两种海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met、Val-Thr-Pro-Tyr的抗氧化活性。应用电子自旋共振的方法（ESR），检测海参肠自溶寡肽对羟基自由基（?OH）、超氧阴离子自由基（O2-?）、1, 1-二苯基-2-三硝基苯肼（DPPH?）自由基的清除情况。同时，采用人白血病细胞Jurkat，考察海参肠自溶寡肽对H2O2诱导的细胞氧化损伤的影响。结果表明，两种海参肠自溶寡肽对?OH、O2-?和DPPH自由基均有很强的清除能力，且呈明显的量效关系。对于不同自由基，Val-Gly-Thr-Val-Glu-Met在相应的浓度下（?OH自由基-1和5 mM、O2-?自由基-1、5和20 mM、DPPH自由基-1和8 mM）清除能力均显著高于Val-Thr-Pro-Tyr（p<0.05）。海参肠自溶寡肽在浓度为5 mM时未能对H2O2诱导的Jurkat细胞DNA氧化损伤起到保护作用，而浓度为20 mM时引起Jurkat细胞死亡。这些结果说明，海参肠自溶寡肽Val-Gly-Thr-Val-Glu-Met和Val-Thr-Pro-Tyr具有自由基清除能力，具有一定的开发价值。

Abstract:

In this paper, the antioxidant activities of oligopeptides (Val-Gly-Thr-Val-Glu-Met and Val-Thr-Pro-Tyr) from sea cucumber guts were studied by measuring the scavenging abilities on hydroxyl radical, superoxide radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical using an ESR spectrometer. Moreover, the influence of oligopeptides on H2O2-induced oxidative damage in Jurkat cells was also investigated. The results showed that two kinds of oligopeptides from sea cucumber guts showed scavenging abilities on ?OH, O2-? and DPPH? in a dose-dependent manner. The scavenging ability of Val-Gly-Thr-Val-Glu-Met was significantly higher than that of Val-Thr-Pro-Tyr at different concentrations (p<0.05). The oligopeptides failed to protect the DNA oxidative damage induced by H2O2 at concentrations of 5 mM in Jurkat cells, while induced cell death at concentration of 20 mM. These results suggest that the oligopeptides from sea cucumber guts (Val-Gly-Thr- Val- Glu-Met,Val-Thr-Pro-Tyr) possess free radical scavenging abilities, and have certain development value.