谷氨酰胺转移酶对明胶-CaCO3矿物质膜成膜性的影响

Effects of Transglutaminase on Properties of Gelatin-CaCO3 Mineral Films

摘要:

本实验通过向明胶-碳酸钙矿物质膜中添加谷氨酰胺转移酶（TGase），研究了谷氨酰胺转移酶对明胶-碳酸钙矿物质膜特性的影响，对谷氨酰胺转移酶处理前后样品进行厚度、质构、水溶性、水蒸气透过系数、扫描电镜（SEM）、流变性、差示热量扫描（DSC）等方法表征。研究结果表明：在成膜溶液中加入谷氨酰胺转移酶（6 U/g）可以使矿物质膜的厚度增加19.69%、成膜液凝胶强度增加17.24%、膜的抗拉强度增加28.05%、断裂伸长率增加21.27%，而水溶性和水蒸气透过率没有显著改变；扫描电镜表明，谷氨酰胺转移酶交联的矿物质膜表面和断面与不加谷氨酰胺转移酶的矿物质膜相比更加粗糙；流变性结果表明，谷氨酰胺转移酶加入后成膜溶液的粘度显著增加；差示热量扫描表明，谷氨酰胺转移酶催化明胶-碳酸钙矿物膜产生了交联。

Abstract:

Transglutaminase (TGase) was added to gelatin-CaCO3 mineral films and its effect on properties of gelatin-CaCO3 mineral films were studied. Thickness, gel strength, tensile strength (TS), elongation at break (EAB), water solubility (WS) and water vapor permeability (WVP) were characterized by scanning electric microscopy (SEM), rheological study and differential scanning calorie (DSC). The results showed that TGase treatment significantly increased (p≤0.05) the thickness, gel strength, TS and EAB of gelatin-CaCO3 mineral films by 19.69%, 17.24%, 28.05% and 21.27%, respectively; TGase treatment had no effect on the WS and WVP values of gelatin-CaCO3 mineral films. The TGase-treated films had rougher surfaces and cross-sections compared with the films without TGase. Rheological study showed that TGase significantly increased the viscosity. DSC confirmed the occurrence of cross-linking induced by TGase.