山莓叶抑制兔离体肠运动活性物质的提取及活性稳定性研究

Inhibitory Effect of Rubus corchorifolius Leaves Extract on the Movement of Isolated Intestinal from Rabbit and Its Active Stability

摘要:

本研究以山莓叶为原料，以兔离体肠运动为模型，80%乙醇提取后采用4种不同极性溶剂进行提取，筛选具有抗腹泻的活性组分，并考察温度、pH、光质、光照时间和脱鞣质处理对粗提物抑制兔离体肠运动活性的影响。结果表明：山莓叶抑制兔离体肠运动的活性物质主要在乙酸乙酯层，其它层提取物的活性大小依次为：石油醚层>水层>正丁醇层>氯仿层；山莓叶醇提物在30 ℃~60 ℃范围内抑制兔离体肠运动活性稳定，高温（90 ℃和120 ℃）条件下离体肠的张力增量变化率较30 ℃处理分别下降了15.43%和32.45%；pH对抑制肠运动活性影响较大，酸性条件下活性最高，其次是中性，碱性条件下抑制其活性，pH 12处理后张力增量变化率仅为11.90%；不同光质和不同光照时间处理后仍具有极显著抑制兔离体肠运动的作用（P<0.01）；脱鞣质处理会使山莓叶醇提物抑制肠运动活性减弱，说明鞣质可能是山莓叶抗腹泻活性成分之一。

Abstract:

Using the movement of isolated intestinal from rabbit as the model, the extraction of anti-diarrhea active components from Rubus corchorifolius leaves and their active stability were investigated. The active components were extracted with 80% ethanol, followed by five different polar solvents. The effects of temperature, pH, light quality, illumination time, and the tannins removal treatment on the activity of the extracts were also studied. The results showed that the active components mainly existed in ethyl acetate layer, the sequence of the extract activity in different solvent layer was petroleum ether layer>water layer>n-butanol layer>chloroform layer. The ethanol extract of Rubus corchorifolius leaves were stable in the range of 30~60 ℃. But at high temperature of 90 ℃ and 120 ℃, the tensional variation percentage of isolated intestinal from rabbit decreased by 15.43% and 32.45%, respectively. pH showed significant influence on the activity, and the highest activity was found under acidic conditions, followed by neutral and alkaline conditions. The tensional variation percentage was only 11.90% at pH 12. The ethanol extract after different light qualities and different illumination time treatments still had high activity (P<0.01), while the activity was decreased by tannin removal treatment, indicating tannin might be one of the components for anti-diarrheal activity in Rubus corchorifolius leaves.