Abstract:Cyclocarya paliurus cell suspension culture was established in this paper, and sodium nitroprusside was added as an extraneous inducer to stimulate the biosynthesis of triterpenic acids. The effects of sodium nitroprusside in terms of concentration, adding time and inducement duration on the cells growth, total triterpenic acids accumulation, and the biosynthesis of five representative triterpenic acids were investigated. The results showed that 10~50 μmol/L sodium nitroprusside had little effect on the growth of Cyclocarya paliuru cells, and little stimulant effect on the biosynthesis of triterpenic acids. 150 μmol/L sodium nitroprusside was suitable for the accumulation of triterpenic acids, and had little effect on the cells growth. High concentrations (200~500 μmol/L) of sodium nitroprusside inhibited the growth seriously. The highest production of triterpenic acids was obtained when 150μmol/L sodium nitroprusside was added at the 8 th day after inoculation and continuous inducement for 4 days. Under this optimal conditions, the production of total triterpenic acids was as higher as 1228.74 mg/L, which was 2.14 times higher than that of the control group. And the output of ursolic acid and oleanolic acid increased more obviously among the five representative triterpenic acids of this cells, which was 2.28 and 2.48 times, respectively, higher than the control group. Our researches indicated that extraneous sodium nitroprusside effectively increased the biosynthesis of triterpenic acids in suspended cultured Cyclocarya paliuru cells.
