补充CO2提高胶球藻C-169生物量和脂肪酸产率的研究

Enhanced Yield of Biomass and Fatty Acids from Coccomyxa subellipsoidea C-169 by CO2 Supplement

摘要:

本研究在Basal和BBM培养基中补充2% CO2或空气（0.04% CO2）光自养培养胶球藻（Coccomyxa subellipsoidea）C-169，系统比较了细胞比生长速率、生物量干重、总脂肪酸含量和产率以及中性脂含量的差异。研究表明，与空气培养相比，在Basal和BBM培养基中补充2% CO2可分别显著提高细胞的比生长速率2.6倍和2.3倍。培养13 d后，Basal培养基中补充2% CO2可获得最高生物量干重达4.67 g/L，显著高于所有其他组（p＜0.05）；而BBM培养基中补充2% CO2可获得最高总脂肪酸含量达37.53%干重，是通入空气时的5倍，脂肪酸产率更是高达77.49 mg/(L?d)，同时中性脂含量（以荧光强度表示）显著高于所有其他组（p＜0.05）。这就说明在Basal培养基中补充CO2有利于细胞生物量的积累；而在BBM培养基中补充CO2更有利于驱动细胞高产脂肪酸和中性脂，其生物量是制备生物柴油的极好原料。

Abstract:

The effects of 2% CO2 supplement or air (contain 0.04% CO2) on cell specific growth rate, biomass dry weight, content and yield of total fatty acids, neutral lipids content of Coccomyxa subellipsoidea C-169 grown autotrophically in Basal and BBM media were investigated in this study. Compared with the results from aeration with air, 2% CO2 supplement significantly increased the cell specific growth rate of 2.6 times in Basal medium and 2.3 times in BBM medium, respectively. After cultured for 13 days, the maximal biomass concentration was obtained at 4.67 g/L by 2% CO2 supplement in Basal medium, which was much higher than other groups (p < 0.05). The highest content of total fatty acids reached 37.53% (DW) by supplying 2% CO2 in BBM medium, which was 5 times higher than that by inletting air. And the yield of total fatty acids was up to 77.49 mg/(L?d) while neutral lipids referred as fluorescence intensity was significantly higher than other groups (p＜0.05). These results indicated that 2% CO2 supplement in Basal medium could promote cells to produce more biomass. In BBM medium, it could increase the yield of fatty acids and neutral lipids in the microalga, which was excellent raw material for biodiesel production.